RabMAb

Anti-Carbonic Anhydrase IX抗体[EPR4151(2)] (ab108351)

概述

  • 产品名称Anti-Carbonic Anhydrase IX抗体[EPR4151(2)]
    参阅全部 Carbonic Anhydrase IX 一抗
  • 描述
    兔单克隆抗体[EPR4151(2)] to Carbonic Anhydrase IX
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues in the extracellular domain in Human Carbonic Anhydrase IX

  • 阳性对照
    • HT-29 and Human stomach lysates. Human stomach and Human renal cell carcinoma tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab108351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/100 - 1/250. Perform antigen retrieval before commencing with IHC staining protocol.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IP.
  • 靶标

    • 功能Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.
    • 组织特异性Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
    • 序列相似性Belongs to the alpha-carbonic anhydrase family.
      Contains 1 alpha-carbonic anhydrase domain.
    • 翻译后修饰Asn-346 bears high-mannose type glycan structures.
    • 细胞定位Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CA-IX antibody
      • CA9 antibody
      • CAH9_HUMAN antibody
      • CAIX antibody
      • Carbonate dehydratase IX antibody
      • Carbonic anhydrase 9 antibody
      • Carbonic anhydrase IX antibody
      • Carbonic dehydratase antibody
      • G250 antibody
      • Membrane antigen MN antibody
      • MN antibody
      • P54/58N antibody
      • pMW1 antibody
      • RCC associated protein G250 antibody
      • RCC-associated antigen G250 antibody
      • Renal cell carcinoma-associated antigen G250 antibody
      see all

    Anti-Carbonic Anhydrase IX antibody [EPR4151(2)] 图像

    • All lanes : Anti-Carbonic Anhydrase IX antibody [EPR4151(2)] (ab108351) at 1/1000 dilution

      Lane 1 : HT-29 cell lysate
      Lane 2 : Human stomach lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 50 kDa
    • Immunohistochemical analysis of paraffin-embedded Human stomach tissue using ab108351 at 1/100
    • Immunohistochemical analysis of paraffin-embedded Human renal cell carcinoma tissue using ab108351 at 1/100

    Anti-Carbonic Anhydrase IX antibody [EPR4151(2)] (ab108351)参考文献

    This product has been referenced in:
    • Gu MJ & Kwon KW Carbonic anhydrase IX expression is associated with favorable prognostic factors in small intestinal carcinoma. J Histochem Cytochem 62:205-10 (2014). IHC . Read more (PubMed: 24217923) »
    • Viola-Villegas NT  et al. Understanding the pharmacological properties of a metabolic PET tracer in prostate cancer. Proc Natl Acad Sci U S A 111:7254-9 (2014). WB . Read more (PubMed: 24785505) »

    See all 3 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Gastric cells)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA buffer, pH 9
    Permeabilization Yes - Triton 0.025%
    Specification Gastric cells
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Jun 03 2015

    Application Western blot
    Loading amount 15 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (Cancer Cell Lines)
    Specification Cancer Cell Lines
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Aug 05 2014

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing (10% gel)
    Sample Human Cell lysate - whole cell (Glioma)
    Specification Glioma
    Blocking step Milk as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Dec 27 2013

    Application Western blot
    Loading amount 30000 cells
    Gel Running Conditions Reduced Denaturing (11)
    Sample Human Cell lysate - whole cell (MCF-7 cells (human breast adenocarcinoma cell line)
    Specification MCF-7 cells (human breast adenocarcinoma cell line
    Treatment 0.5% oxygen for 16hrs
    Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    提交于 Nov 05 2013

    Thank you for contacting us.

    We have several HIF antibodies in our catalog that react with mouse. For example, ab8366: HIF1 alpha, ab199: HIF2 alpha, ab10134: HIF3 alpha, ab465: HIF1 beta. If you could let me know which HIF you are looking fo...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"