Anti-Carbonic Anhydrase IX抗体(ab15086)

概述

  • 产品名称Anti-Carbonic Anhydrase IX抗体
    参阅全部 Carbonic Anhydrase IX 一抗
  • 描述
    兔多克隆抗体to Carbonic Anhydrase IX
  • 经测试应用适用于: IHC-P, Flow Cyt, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide made to a sequence within residues 359-459 of the human protein (C-terminus), accession# Q16790

  • 阳性对照
    • Renal carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab15086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Citrate Buffer (pH 6.0)
Flow Cyt Use at an assay dependent concentration. PubMed: 25269858

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration. PubMed: 20515941Slides were fixed in acetone for 20 minutes.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 49.7 kDa).Can be blocked with Recombinant Human RND1 protein (ab109840).

靶标

  • 功能Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.
  • 组织特异性Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
  • 序列相似性Belongs to the alpha-carbonic anhydrase family.
    Contains 1 alpha-carbonic anhydrase domain.
  • 翻译后修饰Asn-346 bears high-mannose type glycan structures.
  • 细胞定位Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CA-IX antibody
    • CA9 antibody
    • CAH9_HUMAN antibody
    • CAIX antibody
    • Carbonate dehydratase IX antibody
    • Carbonic anhydrase 9 antibody
    • Carbonic anhydrase IX antibody
    • Carbonic dehydratase antibody
    • G250 antibody
    • Membrane antigen MN antibody
    • MN antibody
    • P54/58N antibody
    • pMW1 antibody
    • RCC associated protein G250 antibody
    • RCC-associated antigen G250 antibody
    • Renal cell carcinoma-associated antigen G250 antibody
    see all

Anti-Carbonic Anhydrase IX antibody 图像

  • Formalin fixed paraffin embedded renal carcinoma tissue, positively stained using Ab15086.
  • ab15086 at 1/500 staining dog gastric mucosa tissue sections by IHC-P. The tissue was formaldehyde fixed and a standard citrate buffer was used for anitgen retrieval. The tissue was blocked with 10% serum prior to incubation with the antibody for 15 hours. A biotinylated donkey anti-rabbit IgG (H + L) was used as the secondary.

    See Abreview

  • All lanes : Anti-Carbonic Anhydrase IX antibody (ab15086) at 1 µg/ml

    Lane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 49.7 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

Anti-Carbonic Anhydrase IX antibody (ab15086)参考文献

This product has been referenced in:
  • Friedman GK  et al. ?134.5-deleted HSV-1-expressing human cytomegalovirus IRS1 gene kills human glioblastoma cells as efficiently as wild-type HSV-1 in normoxia or hypoxia. Gene Ther 22:348-55 (2015). Read more (PubMed: 25427614) »
  • Verduzco D  et al. Intermittent hypoxia selects for genotypes and phenotypes that increase survival, invasion, and therapy resistance. PLoS One 10:e0120958 (2015). Read more (PubMed: 25811878) »

See all 35 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Sample Human Tissue sections (Colorectal tumors)
Specification Colorectal tumors
Permeabilization Yes - 1xPBS+0.3%t-X-100
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 09 2014

Thank you for contacting us.

We have several HIF antibodies in our catalog that react with mouse. For example, ab8366: HIF1 alpha, ab199: HIF2 alpha, ab10134: HIF3 alpha, ab465: HIF1 beta. If you could let me know which HIF you are looking fo...

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Thank you for contacting us. Unfortunately we do not have any kit corresponding to the detection of the CAIX protein. However, I would suggest to develop an ELISA assay using the CAIX protein ab114200 that would be coated on the plate. A control ...

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Thank you for your patient. Unfortunately there is no number that I can provide to you as it is impossible to know for sure how many slides you will be able to stain with a vial. This will depend on the experimental conditions, the samples and how high...

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Thank you for contacting us. The concentration which is recommended for performing IHC-P with Anti-Carbonic Anhydrase IX antibody (ab15086) is 1/1000-1/2000. Taking the more concentrated range, I have estimated that from the 100µl provided (and inc...

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Thank you for your reply. It seems from your description that you are doing everything fine. If I understand correctly, you are using the peptide at 1 ug/mL and the antibody at 0.1 ug/mL (1/10,000 dilution). At same volumes, the peptid...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Soft Tissue Sarcoma, Gastric Mucosa)
Specification Soft Tissue Sarcoma, Gastric Mucosa
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

提交于 Dec 13 2006

Thank you for your enquiry. I enquired with our source for this antibody and there have not been any reports of this problem where the antibody is staining muscle. Ab15086 was characterized for use in IHC with renal carcinoma tissue and I would recomme...

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Thank you for your enquiry. The immunogen is a synthetic peptide made to a sequence within residues 359-459 of the human protein (the C-terminus), accession# Q16790. The exact sequence is proprietary information and cannot be disclosed. I hope...

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Thank you for your enquiry and your interest in our products. According to Swiss-Prot database this Carbonic Anhydrase IX can be found on the surface microvilli and in the nucleus.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"