Anti-Carbonic Anhydrase IX抗体[2D3] (ab107257)

概述

  • 产品名称Anti-Carbonic Anhydrase IX抗体[2D3]
    参阅全部 Carbonic Anhydrase IX 一抗
  • 描述
    小鼠单克隆抗体[2D3] to Carbonic Anhydrase IX
  • 经测试应用适用于: Flow Cyt, WB, IHC-P, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified recombinant fragment of human Carbonic Anhydrase IX expressed in E. Coli. (Q16790).

  • 阳性对照
    • Hela and A549 cell lysate; lung and colonic tissues and NTERA-2 cells.

性能

应用

Our Abpromise guarantee covers the use of ab107257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/2000. Predicted molecular weight: 50 kDa.
IHC-P 1/200 - 1/1000.
ICC/IF 1/200 - 1/1000.
ELISA 1/10000.

靶标

  • 功能Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.
  • 组织特异性Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
  • 序列相似性Belongs to the alpha-carbonic anhydrase family.
    Contains 1 alpha-carbonic anhydrase domain.
  • 翻译后修饰Asn-346 bears high-mannose type glycan structures.
  • 细胞定位Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CA-IX antibody
    • CA9 antibody
    • CAH9_HUMAN antibody
    • CAIX antibody
    • Carbonate dehydratase IX antibody
    • Carbonic anhydrase 9 antibody
    • Carbonic anhydrase IX antibody
    • Carbonic dehydratase antibody
    • G250 antibody
    • Membrane antigen MN antibody
    • MN antibody
    • P54/58N antibody
    • pMW1 antibody
    • RCC associated protein G250 antibody
    • RCC-associated antigen G250 antibody
    • Renal cell carcinoma-associated antigen G250 antibody
    see all

Anti-Carbonic Anhydrase IX antibody [2D3] 图像

  • All lanes : Anti-Carbonic Anhydrase IX antibody [2D3] (ab107257) at 1/500 dilution

    Lane 1 : Hela Cell lysate
    Lane 2 : A549 Cell Lysate


    Predicted band size : 50 kDa
  • Flow cytometric analysis of NTERA-2 cells using Carbonic Anhydrase IX mouse ab107257 in green and negative control in purple.
  • Immunohistochemical analysis of paraffin-embedded lung tissues (left) and colonic tissues (right) using 1/200 ab107257 with DAB staining.

Anti-Carbonic Anhydrase IX antibody [2D3] (ab107257)参考文献

This product has been referenced in:
  • Schweiger T  et al. Carbonic anhydrase IX is associated with early pulmonary spreading of primary colorectal carcinoma and tobacco smoking. Eur J Cardiothorac Surg N/A:N/A (2013). Human . Read more (PubMed: 24321995) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Gastric cells)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA buffer, pH 9
Permeabilization Yes - Triton 0.025%
Specification Gastric cells
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 03 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Glioblastoma primary cells)
Loading amount 50 µg
Specification Glioblastoma primary cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Nov 21 2012

Thank you for contacting us.
I am sorry that the vial you received did not contain the full amount of the product and I apologize for the inconvenience. I have issued a free of charge replacement vial with the order number of 1112020.
Please ...

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Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

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Thank you for contacting us. Unfortunately we do not have any kit corresponding to the detection of the CAIX protein. However, I would suggest to develop an ELISA assay using the CAIX protein ab114200 that would be coated on the plate. A control ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"