Anti-Carbonic Anhydrase III抗体- N-terminal (ab85366)


  • 产品名称Anti-Carbonic Anhydrase III抗体- N-terminal
    参阅全部 Carbonic Anhydrase III 一抗
  • 描述
    兔多克隆抗体to Carbonic Anhydrase III - N-terminal
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide derived from the N-terminal region of Human Carbonic Anhydrase III (NP_005172), conjugated to KLH

  • 阳性对照
    • HepG2 cell line lysate; Mouse kidney tissue lysate.




Our Abpromise guarantee covers the use of ab85366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50 - 1/100. Predicted molecular weight: 30 kDa.
ELISA 1/1000.
ICC/IF Use a concentration of 5 µg/ml.


  • 功能Reversible hydration of carbon dioxide.
  • 组织特异性Muscle specific.
  • 序列相似性Belongs to the alpha-carbonic anhydrase family.
  • 发展阶段At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.
  • 翻译后修饰S-glutathionylated in hepatocytes under oxidative stress.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CA III antibody
    • CA-III antibody
    • Ca3 antibody
    • CAH3_HUMAN antibody
    • CAIII antibody
    • Car3 antibody
    • Carbonate dehydratase III antibody
    • Carbonic anhydrase 3 antibody
    • Carbonic anhydrase III antibody
    • Carbonic anhydrase III muscle specific antibody
    • EC antibody
    • Epididymis secretory sperm binding protein Li 167mP antibody
    • HEL S 167mP antibody
    see all

Anti-Carbonic Anhydrase III antibody - N-terminal 图像

  • Anti-Carbonic Anhydrase III antibody - N-terminal (ab85366) at 1/50 dilution + HepG2 cell lysate at 35 µg

    Predicted band size : 30 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • Anti-Carbonic Anhydrase III antibody - N-terminal (ab85366) at 1/50 dilution + Mouse kidney tissue lysate at 35 µg

    Predicted band size : 30 kDa
    Observed band size : 30 kDa
  • ICC/IF image of ab85366 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85366, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Carbonic Anhydrase III antibody - N-terminal (ab85366)参考文献

ab85366 has not yet been referenced specifically in any publications.

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