Anti-Carbonic Anhydrase III抗体(ab118428)

概述

  • 产品名称Anti-Carbonic Anhydrase III抗体
    参阅全部 Carbonic Anhydrase III 一抗
  • 描述
    兔多克隆抗体to Carbonic Anhydrase III
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Recombinant Fragment
    预测可用于: Rabbit, Cow, Dog, Pig, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Carbonic Anhydrase III.

  • 阳性对照
    • This antibody gave a positive signal in Recombinant Carbonic anhydrase 3 protein as well as the following tissue lysates: Human Skeletal Muscle; Mouse Skeletal Muscle; Rat Skeletal Muscle. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab118428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 29 kDa).
ICC/IF Use a concentration of 10 µg/ml.

靶标

Anti-Carbonic Anhydrase III antibody 图像

  • ICC/IF image of ab118428 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab118428 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-Carbonic Anhydrase III antibody (ab118428) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 3 : Skeletal Muscle (Rat) Tissue Lysate
    Lane 4 : Carbonic Anhydrase III protein (ab50779)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes

Anti-Carbonic Anhydrase III antibody (ab118428)参考文献

ab118428 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab118428.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"