Anti-Carbonic Anhydrase II抗体(Biotin) (ab34675)

概述

  • 产品名称
    Anti-Carbonic Anhydrase II抗体(Biotin)
    参阅全部 Carbonic Anhydrase II 一抗
  • 描述
    兔多克隆抗体to Carbonic Anhydrase II (Biotin)
  • 偶联物
    Biotin
  • 经测试应用
    适用于: WB, Dot blot, Immunomicroscopy, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Carbonic Anhydrase II from Human Erythrocytes.

  • 常规说明

    10-20 Biotin molecules per rabbit IgG molecule.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.01% (w/v) Sodium Azide
    Constituents: 10mg/ml BSA (IgG and Protease free), 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 纯化说明
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against buffer.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab34675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
Dot blot
Immunomicroscopy
ELISA
  • 应用说明
    Dot: Use at an assay dependent dilution.
    ELISA: 1/4000 - 1/20000. This antibody has been assayed against 1.0µg of Carbonic Anhydrase II in a standard capture ELISA using Peroxidase conjugated streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at RT.
    IM: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 29 kDa.
    Suitable for other antibody based assays using streptavidin or avidin conjugates.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye.
    • 疾病相关
      Defects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3) [MIM:259730]; also known as osteopetrosis with renal tubular acidosis, carbonic anhydrase II deficiency syndrome, Guibaud-Vainsel syndrome or marble brain disease. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB3 is associated with renal tubular acidosis, cerebral calcification (marble brain disease) and in some cases with mental retardation.
    • 序列相似性
      Belongs to the alpha-carbonic anhydrase family.
    • 细胞定位
      Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CA 2 antibody
      • CA II antibody
      • CA-II antibody
      • Ca2 antibody
      • CAC antibody
      • CAH2_HUMAN antibody
      • CAII antibody
      • Car 2 antibody
      • Car2 antibody
      • Carbonate dehydratase II antibody
      • Carbonic anhydrase 2 antibody
      • Carbonic anhydrase B antibody
      • Carbonic anhydrase C antibody
      • Carbonic anhydrase C, formerly antibody
      • Carbonic anhydrase II antibody
      • Carbonic dehydratase antibody
      • epididymis luminal protein 76 antibody
      • Epididymis secretory protein Li 282 antibody
      • HEL-76 antibody
      • HEL-S-282 antibody
      see all

    实验方案

    Anti-Carbonic Anhydrase II antibody (Biotin) (ab34675)参考文献

    ab34675 has not yet been referenced specifically in any publications.

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