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Anti-Carbonic Anhydrase II抗体(ab82559)

概述

  • 产品名称Anti-Carbonic Anhydrase II抗体
    参阅全部 Carbonic Anhydrase II 一抗
  • 描述
    兔多克隆抗体to Carbonic Anhydrase II
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Sheep, Rabbit, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Carbonic Anhydrase II.

    (Peptide available as ab92423.)

  • 阳性对照
    • This antibody gave a positive signal in HEK293 whole cell lysate and in the following human tissue lysates: Bone Marrow; Small Intestine; Kidney.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab82559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

靶标

  • 功能Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye.
  • 疾病相关Defects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3) [MIM:259730]; also known as osteopetrosis with renal tubular acidosis, carbonic anhydrase II deficiency syndrome, Guibaud-Vainsel syndrome or marble brain disease. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB3 is associated with renal tubular acidosis, cerebral calcification (marble brain disease) and in some cases with mental retardation.
  • 序列相似性Belongs to the alpha-carbonic anhydrase family.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CA 2 antibody
    • CA II antibody
    • CA-II antibody
    • Ca2 antibody
    • CAC antibody
    • CAH2_HUMAN antibody
    • CAII antibody
    • Car 2 antibody
    • Car2 antibody
    • Carbonate dehydratase II antibody
    • Carbonic anhydrase 2 antibody
    • Carbonic anhydrase B antibody
    • Carbonic anhydrase C antibody
    • Carbonic anhydrase C, formerly antibody
    • Carbonic anhydrase II antibody
    • Carbonic dehydratase antibody
    • epididymis luminal protein 76 antibody
    • HEL-76 antibody
    see all

Anti-Carbonic Anhydrase II antibody 图像



  • Predicted band size : 29 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Carbonic Anhydrase II knockout HAP1 cell lysate (20 µg)
    Lane 3: A431 cell lysate (20 µg)
    Lane 4: HEk293 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab82559 observed at 30 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab82559 was shown to specifically react with Carbonic Anhydrase II when Carbonic Anhydrase II knockout samples were used. Wild-type and Carbonic Anhydrase II knockout samples were subjected to SDS-PAGE. ab82559 and ab8245 (loading control to GAPDH) were diluted at 1 μg/mL and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-Carbonic Anhydrase II antibody (ab82559) at 1 µg/ml

    Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 29 kDa


    Exposure time : 1 minute

Anti-Carbonic Anhydrase II antibody (ab82559)参考文献

ab82559 has not yet been referenced specifically in any publications.

Product Wall

Application Blocking
Sample Cow Purified protein (bovine carbonic anhydrases from erythrocytes)
Specification bovine carbonic anhydrases from erythrocytes
Username

Miss. Radhika Mehta

Verified customer

提交于 Oct 20 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"