重组
RabMAb

Anti-Carbonic Anhydrase I抗体[EPR5193] (ab108367)

概述

  • 产品名称
    Anti-Carbonic Anhydrase I抗体[EPR5193]
    参阅全部 Carbonic Anhydrase I 一抗
  • 描述
    兔单克隆抗体[EPR5193] to Carbonic Anhydrase I
  • 经测试应用
    适用于: WB, ICC, IHC-Frmore details
    不适用于: Flow Cyt,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human, Zebrafish
  • 免疫原

    A synthetic peptide corresponding to C-terminal residues of Human Carbonic Anhydrase 1

  • 阳性对照
    • K562 and TF1 cell lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Alternative versions available:

    Anti-Carbonic Anhydrase I antibody (HRP) [EPR5193] (ab202929)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab108367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Predicted molecular weight: 29 kDa.
ICC 1/1000 - 1/5000.
IHC-Fr 1/200.
  • 应用说明
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • 靶标

    图片

    • ab108367 staining Carbonic Anhydrase I in zebrafish retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 5% BSA in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab108367 at 1/200 in blocking solution for 16h at 4°C. An alexa 488 conjugated goat anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. Carbonic Anhydrase I expression can be observed in horizontal cells and Muller cells(in green).

      See Abreview

    • All lanes : Anti-Carbonic Anhydrase I antibody [EPR5193] (ab108367) at 1/10000 dilution

      Lane 1 : K562 cell lysates
      Lane 2 : TF1 cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 29 kDa

    文献

    This product has been referenced in:

    See all 2 Publications for this product

    客户评价及客户问答

    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (12.5% gel)
    Sample
    Human Cell lysate - whole cell (293T)
    Specification
    293T
    Treatment
    CA1-3xflag transfected
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Jan 30 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample
    Zebrafish Tissue sections (Retina, Muller cells and Horizontal cells)
    Specification
    Retina, Muller cells and Horizontal cells
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Username

    Dr. Ryan Macdonald

    Verified customer

    提交于 Jul 03 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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