CAPZB overexpression 293T裂解液(全细胞) (ab94201)

概述

  • 产品名称CAPZB overexpression 293T裂解液(全细胞)
  • 描述
    CAPZB overexpression裂解液
  • 常规说明ab94201 is a 293T cell transfected lysate in which Human CAPZB has been transiently over-expressed using a pCMV-CAPZB plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Function: F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. Similarity: Belongs to the F-actin-capping protein beta subunit family.

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应用

Our Abpromise guarantee covers the use of ab94201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

CAPZB overexpression 293T lysate (whole cell) 图像

  • ab94201 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-CAPZB antibody (ab54930) at 1/500 dilution

    Lane 1 : CAPZB overexpression 293T lysate (whole cell) (ab94201)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

CAPZB overexpression 293T lysate (whole cell) (ab94201)参考文献

ab94201 has not yet been referenced specifically in any publications.

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