Anti-CAPS1抗体(ab32787)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to CAPS1
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Cow, Human, Xenopus laevis
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Rat CAPS1.

    (Peptide available as ab32786.)

  • 阳性对照
    • ab32787 gave a positive result in rat and mouse brain tissue lysates. CAPS1 is known not to be expressed in heart (Speidel et al, J. Biol. Chem., Vol. 278, Issue 52, 52802-52809. PMID: 14530279). Mouse heart tissue lysate can be used as a negative control for ab32787.

性能

应用

Our Abpromise guarantee covers the use of ab32787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明
    IHC-P: 1/300.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 146 kDa (predicted molecular weight: 146 kDa). Can be blocked with CAPS1 peptide (ab32786). Block in 5% milk


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      CAPS1 (Ca2+-dependent activator protein for secretion) belongs to the CAPS/Cadps family which consists of two members, CAPS1 and CAPS2. The CAPS family proteins are involved in the secretion of different secretory substances in developing and postnatal brains. In addition CAPS1 regulates catecholamine release from neuroendocrine cells and is expressed predominantly in the brain.
    • 细胞定位
      Cytoplasmic
    • 数据库链接
    • 别名
      • CADPS antibody
      • CADPS1 antibody
      • Calcium dependent secretion activator 1 antibody
      • Calcium-dependent activator protein for secretion 1 antibody
      • CAPS antibody
      • KIAA1121 antibody
      • rCAPS antibody
      see all

    图片

    • All lanes : Anti-CAPS1 antibody (ab32787) at 1 µg/ml

      Lane 1 : Brain (Rat) Tissue Lysate at 10 µg
      Lane 2 : Brain (Mouse) Tissue Lysate at 10 µg
      Lane 3 : Heart (Mouse) Tissue Lysate at 10 µg
      Lane 4 : Brain (Rat) Tissue Lysate with Rat CAPS1 peptide (ab32786) at 1 µg/ml
      Lane 5 : Brain (Mouse) Tissue Lysate at 10 µg with Rat CAPS1 peptide (ab32786) at 1 µg/ml
      Lane 6 : Heart (Mouse) Tissue Lysate at 10 µg with Rat CAPS1 peptide (ab32786) at 1 µg/ml

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 146 kDa
      Observed band size : 146 kDa

    文献

    ab32787 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (brain sections)
    Specification
    brain sections
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Username

    Dr. Sophie Pezet

    Verified customer

    提交于 Aug 25 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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