概述

  • 产品名称Anti-CAPON抗体
    参阅全部 CAPON 一抗
  • 描述
    兔多克隆抗体to CAPON
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human CAPON.

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Human brain; Human spinal cord.

性能

应用

Our Abpromise guarantee covers the use of ab90854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 56 kDa).

靶标

  • 功能Adapter protein involved in neuronal nitric-oxide (NO) synthesis regulation via its association with nNOS/NOS1. The complex formed with NOS1 and synapsins is necessary for specific NO and synapsin functions at a presynaptic level. Mediates an indirect interaction between NOS1 and RASD1 leading to enhance the ability of NOS1 to activate RASD1. Competes with DLG4 for interaction with NOS1, possibly affecting NOS1 activity by regulating the interaction between NOS1 and DLG4.
  • 序列相似性Contains 1 PID domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 6330408P19Rik antibody
    • C terminal PDZ domain ligand of neuronal nitric oxide synthase (CAPON) antibody
    • C terminal PDZ domain ligand of neuronal nitric oxide synthase antibody
    • C terminal PDZ ligand of neuronal nitric oxide synthase protein antibody
    • C-terminal PDZ ligand of neuronal nitric oxide synthase protein antibody
    • CAPON antibody
    • CAPON_HUMAN antibody
    • Carboxyl terminal PDZ ligand of neuronal nitric oxide synthase protein antibody
    • Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein antibody
    • Ligand of neuronal nitric oxide synthase with carboxyl terminal PDZ domain antibody
    • MGC138500 antibody
    • Nitric oxide synthase 1 (neuronal) adaptor protein antibody
    • Nitric oxide synthase 1 adaptor protein antibody
    • Nos1ap antibody
    see all

Anti-CAPON antibody 图像

  • All lanes : Anti-CAPON antibody (ab90854) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human spinal cord tissue lysate - total protein (ab29188)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 24 kDa,35 kDa,45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

Anti-CAPON antibody (ab90854)参考文献

This product has been referenced in:
  • Altamirano F  et al. Whole body periodic acceleration is an effective therapy to ameliorate muscular dystrophy in mdx mice. PLoS One 9:e106590 (2014). WB ; Mouse . Read more (PubMed: 25181488) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (Rat cortical neurons)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 30 µg
Specification Rat cortical neurons
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
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Verified customer

提交于 Aug 11 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T cell)
Loading amount 50 µg
Specification 293T cell
Treatment CoCl2 400 µM for 24hrs
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Jun 23 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"