概述

  • 产品名称Anti-CAP2抗体[EPR6377]
    参阅全部 CAP2 一抗
  • 描述
    兔单克隆抗体[EPR6377] to CAP2
  • 经测试应用适用于: WB, IP, Flow Cytmore details
    不适用于: ICC or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to residues from Human CAP2.

  • 阳性对照
    • HepG2, SH-SY5Y, Human heart lysates; permeabilized SH-SY5Y cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab124692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC or IHC-P.
  • 靶标

    • 功能May have a regulatory bifunctional role.
    • 序列相似性Belongs to the CAP family.
      Contains 1 C-CAP/cofactor C-like domain.
    • 细胞定位Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 2810452G09Rik antibody
      • adenylate cyclase associated protein 2 antibody
      • Adenylyl cyclase associated protein 2 antibody
      • Adenylyl cyclase-associated protein 2 antibody
      • CAP 2 antibody
      • CAP adenylate cyclase associated protein 2 antibody
      • CAP adenylate cyclase-associated protein 2 antibody
      • CAP, adenylate cyclase associated protein, 2 (yeast) antibody
      • cap2 antibody
      • CAP2_HUMAN antibody
      see all

    Anti-CAP2 antibody [EPR6377] 图像

    • All lanes : Anti-CAP2 antibody [EPR6377] (ab124692) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : Human heart tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 53 kDa
    • ab124692, at 1/100, staining CAP2 in permeabilized SH-SY5Y cells by flow cytometry (red), compared with a negative control Rabbit IgG antibody (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-CAP2 antibody [EPR6377] (ab124692)参考文献

    This product has been referenced in:
    • Masugi Y  et al. Overexpression of adenylate cyclase-associated protein 2 is a novel prognostic marker in malignant melanoma. Pathol Int 65:627-34 (2015). IHC-P ; Human . Read more (PubMed: 26374196) »

    See 1 Publication for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Duodenum)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01M sodium citrate buffer
    Permeabilization No
    Specification Duodenum
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 20°C
    Fixative Formaldehyde
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    提交于 Jan 12 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"