Anti-CAP1抗体(ab96354)

概述

  • 产品名称Anti-CAP1抗体
    参阅全部 CAP1 一抗
  • 描述
    兔多克隆抗体to CAP1
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Recombinant fragment corresponding to a region within amino acids 266-462 of Human CAP1 (NP_001099000)

  • 阳性对照
    • 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji cell lines. Paraffin-embedded DLD1 xenograft. This antibody gave a positive result when used in the following formaldehyde/methanol fixed cell lines: PANC-1.

性能

应用

Our Abpromise guarantee covers the use of ab96354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.
IHC-P 1/100 - 1/500.
ICC/IF Use a concentration of 5 µg/ml.

靶标

Anti-CAP1 antibody 图像

  • ICC/IF image of ab96354 stained PANC-1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96354 at 5 ug/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-CAP1 antibody (ab96354) at 1/1000 dilution

    Lane 1 : Hep G2 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 52 kDa
  • ab96354 at 1/500 dilution staining paraffin-embedded DLD1 xenograft

Anti-CAP1 antibody (ab96354)参考文献

ab96354 has not yet been referenced specifically in any publications.

Product Wall

I am very pleased to hear you would like to accept our offer and test ab96354 in mouse. This code will give you $337 off your next order before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"