Anti-CAP1抗体(ab88446)
Key features and details
- Mouse polyclonal to CAP1
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CAP1抗体
参阅全部 CAP1 一抗 -
描述
小鼠多克隆抗体to CAP1 -
宿主
Mouse -
经测试应用
适用于: WB, Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human CAP1 aa 1-500. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q01518 -
阳性对照
- Human colon membrane lysate A-431 membrane lysate Transfected 293T membrane lysate HeLa cells Methanol treated human HeLa cells Methanol treated human promonocytic THP-1 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab88446于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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Flow Cyt |
Use at an assay dependent concentration.
ab37355 - Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.
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ICC/IF |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
Flow Cyt
Use at an assay dependent concentration. ab37355 - Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.
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ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Directly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity. -
序列相似性
Belongs to the CAP family.
Contains 1 C-CAP/cofactor C-like domain. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 10487 Human
- Omim: 601896 Human
- SwissProt: Q01518 Human
- Unigene: 370581 Human
- Unigene: 713078 Human
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别名
- Adenylyl cyclase associated protein 1 antibody
- Adenylyl cyclase-associated protein 1 antibody
- CAP 1 antibody
see all
图片
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Anti-CAP1 antibody (ab88446) at 1 µg/ml + Human colon tissue at 50 µg
Secondary
Goat Anti-Mouse IgG at 1/2500 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa -
Anti-CAP1 antibody (ab88446) at 1 µg/ml + A-431 cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG at 1/2500 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa -
All lanes : Anti-CAP1 antibody (ab88446) at 1 µg/ml
Lane 1 : Transfected 293T cell line
Lane 2 : Non-transfected cell line
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG at 1/2500 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa -
Immunofluorescence image staining human CAP1 in HeLa cells, using ab88446 at 10µg/ml.
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FACS analysis of (a) negative control unstained, (b) negative control goat anti-mouse-FITC stained and (c) ab88446 stained methanol-treated human HeLa cells.
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FACS analysis of (a) negative control unstained, (b) negative control goat anti-mouse-FITC stained and (c) ab88446 stained methanol-treated human promonocytic THP-1 cells.
实验方案
数据表及文件
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Datasheet download
文献 (2)
ab88446 被引用在 2 文献中.
- Jin ZL et al. AIP1 and Cofilin control the actin dynamics to modulate the asymmetric division and cytokinesis in mouse oocytes. FASEB J 34:11292-11306 (2020). PubMed: 32602619
- Jin ZL et al. CAP1-mediated actin cycling via ADF/cofilin proteins is essential for asymmetric division in mouse oocytes. J Cell Sci 131:N/A (2018). PubMed: 30404832