Anti-Cannabinoid Receptor II抗体(ab3560)


  • 产品名称Anti-Cannabinoid Receptor II抗体
    参阅全部 Cannabinoid Receptor II 一抗
  • 描述
    兔多克隆抗体to Cannabinoid Receptor II
  • 经测试应用适用于: ICC/IF, IP, IHC-Fr, Flow Cyt, IHC-P, ICC, WBmore details
  • 种属反应性
    与反应: Mouse, Hamster, Human, Non human primates
  • 免疫原

    Fusion protein corresponding to Human Cannabinoid Receptor II aa 1-33 (N terminal).

  • 阳性对照
    • Human spinal cord lysate IF/ICC: SKNSH cell line.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol, 0.1% BSA, 49% PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • Primary antibody说明Cannabinoids exert their well known physiological effects through two G protein coupled receptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors have been shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated protein kinase, MAPK. CB1 receptors also modulate ion channels through direct G-protein interactions. Delta 9-tetrahydrocannibinol and related ligands likely exert their psychoactive effects by inhibiting presynaptic N- and P / Q type calcium channels. CB2 is thought to function primarily in the immune system although it has been suggested to be present in the central nervous system, including the retina.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab3560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 22387618
IHC-Fr 1/100.
Flow Cyt 1/50.
IHC-P 1/100.
ICC 1/100.
WB 1/250 - 1/2500. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).


  • 功能Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis.
  • 组织特异性Preferentially expressed in cells of the immune system with higher expression in B cells and NK cells (at protein level). Expressed in skin in suprabasal layers and hair follicles (at protein level). Highly expressed in tonsil and to a lower extent in spleen, peripheral blood mononuclear cells, and thymus. PubMed:14657172 could not detect expression in normal brain. Expressed in brain by perivascular microglial cells and dorsal root glanglion sensory neurons (at protein level).
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 翻译后修饰Constitutively phosphorylated on Ser-352; phosphorylation increases cell internalization and desensitizes the receptor.
  • 细胞定位Cell membrane. Cell projection > dendrite. Perikaryon. Localizes to apical dendrite of pyramidal neurons.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cannabinoid receptor 2 antibody
    • Cannabinoid receptor 2 macrophage antibody
    • CB 2 antibody
    • CB-2 antibody
    • CB2 antibody
    • CNR2 antibody
    • CNR2_HUMAN antibody
    • CNRII antibody
    • CX 5 antibody
    • CX5 antibody
    • hCB2 antibody
    • testis-dominant CNR2 isoform CB2 antibody
    see all

Anti-Cannabinoid Receptor II antibody 图像

  • ICC/IF image of ab3560 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3560, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Cannabinoid Receptor II antibody (ab3560)参考文献

This product has been referenced in:
  • Kleyer J  et al. Cannabinoid receptor trafficking in peripheral cells is dynamically regulated by a binary biochemical switch. Biochem Pharmacol 83:1393-412 (2012). Flow Cyt, IP ; Human . Read more (PubMed: 22387618) »

See 1 Publication for this product

Product Wall

Application ELISA
Sample Human Recombinant protein (H00001269-P01 (Novusbio), CNR2 recombinant protein)
Specification H00001269-P01 (Novusbio), CNR2 recombinant protein
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Type Indirect

Miss. Carina Kozmus

Verified customer

提交于 Nov 25 2015

Application Flow Cytometry
Sample Human Cell (PBMC)
Permeabilization No
Gating Strategy Lymphocytes B (CD19+)
Specification PBMC
Preparation Cell harvesting/tissue preparation method: ficoll
Sample buffer: PBS 1%BSA 0.01%NaN3

Abcam user community

Verified customer

提交于 Oct 16 2015

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We have following CB1 and CB2 available. These are testes in flow cytometry and are fully guaranteed.


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This product has been successfully tested in IHC with frozen sections. We have not tested the product with paraffin sections (formalin-fixed). It may work, but I would definitely use an antigen retrieval step. We have protocols listed on our website...

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