Anti-Cannabinoid Receptor I抗体(ab107817)


  • 产品名称Anti-Cannabinoid Receptor I抗体
    参阅全部 Cannabinoid Receptor I 一抗
  • 描述
    兔多克隆抗体to Cannabinoid Receptor I
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Rabbit, Horse, Cow, Dog, Human, Pig, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Mouse Cannabinoid Receptor I.

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Mouse Forebrain; Rat Brain; Rat Forebrain. It also gave a positive signal in mouse brain FFPE tissue sections with Immunohistochemistry.



Our Abpromise guarantee covers the use of ab107817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


Anti-Cannabinoid Receptor I antibody 图像

  • IHC image of ab107817 staining in mouse brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab107817, 1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • All lanes : Anti-Cannabinoid Receptor I antibody (ab107817) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Forebrain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate
    Lane 4 : Rat Forebrain Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-Cannabinoid Receptor I antibody (ab107817)参考文献

ab107817 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab107817.
Please use the links above to contact us or submit feedback about this product.