Anti-cAMP Protein Kinase Catalytic subunit抗体(ab76513)

概述

  • 产品名称Anti-cAMP Protein Kinase Catalytic subunit抗体
    参阅全部 cAMP Protein Kinase Catalytic subunit 一抗
  • 描述
    小鼠单克隆抗体to cAMP Protein Kinase Catalytic subunit
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Recombinant Fragment
    预测可用于: Human
  • 免疫原

    Recombinant fragment corresponding to Human cAMP Protein Kinase Catalytic subunit aa 1-121.

应用

Our Abpromise guarantee covers the use of ab76513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明ELISA: Use at a concentration of 10 µg/ml. Detection limit as a capture antibody: 0.1 ng/ml
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 40 kDa. Note: the antibody was only proven to be reactive against recombinant PKA.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and anti-invasive effects of alpha-difluoromethylornithine in breast cancer cells when activated. RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca(2+), leading to reduced amplitude and increased frequency of store overload-induced Ca(2+) release (SOICR) characterized by an increased rate of Ca(2+) release and propagation velocity of spontaneous Ca(2+) waves, despite reduced wave amplitude and resting cytosolic Ca(2+). PSMC5/RPT6 activation by phosphorylation stimulates proteasome. Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation. NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation. May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT). Phosphorylates APOBEC3G and AICDA. Isoform 2 phosphorylates and activates ABL1 in sperm flagellum to promote spermatozoa capacitation.
    • 组织特异性Isoform 1 is ubiquitous. Isoform 2 is sperm-specific and is enriched in pachytene spermatocytes but is not detected in round spermatids.
    • 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 protein kinase domain.
    • 翻译后修饰Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively.
      Autophosphorylated. Phosphorylation is enhanced by vitamin K(2). Phosphorylated on threonine and serine residues. Phosphorylation on Thr-198 is required for full activity.
      Phosphorylated at Tyr-331 by activated receptor tyrosine kinases EGFR and PDGFR; this increases catalytic efficienncy.
    • 细胞定位Cytoplasm. Cell membrane. Nucleus. Mitochondrion. Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm. Distributed throughout the cytoplasm in meiotically incompetent oocytes. Associated to mitochondrion as meiotic competence is acquired. Aggregates around the germinal vesicles (GV) at the immature GV stage oocytes and Cell projection, cilium, flagellum. Expressed in the midpiece region of the sperm flagellum.
    • Information by UniProt
    • 数据库链接
    • 别名
      • cAMP dependent protein kinase alpha catalytic subunit antibody
      • cAMP dependent protein kinase beta catalytic subunit antibody
      • cAMP dependent protein kinase catalytic beta subunit isoform 4ab antibody
      • cAMP dependent protein kinase catalytic subunit alpha antibody
      • cAMP dependent protein kinase catalytic subunit alpha, isoform 1 antibody
      • cAMP dependent protein kinase catalytic subunit beta antibody
      • cAMP-dependent protein kinase catalytic subunit alpha antibody
      • KAPCA_HUMAN antibody
      • PKA C alpha antibody
      • PKA C beta antibody
      • PKA C-alpha antibody
      • PKACA antibody
      • PKACB antibody
      • PPNAD4 antibody
      • PRKACA antibody
      • PRKACAA antibody
      • PRKACB antibody
      • Protein kinase A catalytic subunit alpha antibody
      • Protein kinase A catalytic subunit antibody
      • Protein kinase A catalytic subunit beta antibody
      • Protein kinase, cAMP dependent, catalytic, alpha antibody
      • Protein kinase, cAMP dependent, catalytic, beta antibody
      see all

    Anti-cAMP Protein Kinase Catalytic subunit antibody 图像

    • Anti-cAMP Protein Kinase Catalytic subunit antibody (ab76513) at 5 µg/ml + immunogen (GST-tagged recombinant fragment) at 0.2 µg/ml

      Secondary
      Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution

      Predicted band size : 40 kDa
      Observed band size : 40 kDa
      MW of GST tag alone is 26 kDa.
    • Use of ab76513 as a capture antibody for ELISA.
      Detection limit is approximately 0.1ng/ml.

    Anti-cAMP Protein Kinase Catalytic subunit antibody (ab76513)参考文献

    ab76513 has not yet been referenced specifically in any publications.

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