概述

  • 产品名称cAMP Direct Immunoassay试剂盒
    参阅全部 cAMP 试剂盒
  • 测试
    100 x 1 assay
  • 样品类型
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • 检测类型Quantitative
  • 灵敏度
    > 0.02 µM
  • 范围
    0.02 µM - 2 µM
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: A wide range of mammals
  • 产品概述

    cAMP Assay Kit (ab65355) provides a direct competitive immunoassay for sensitive and quantitative determination of cAMP level in biological samples. The kit utilizes recombinant Protein G coated 96-well plate to efficiently anchor cAMP polyclonal antibody on to the plate. cAMP-HRP conjugate directly competes with cAMP from sample binding to the cAMP antibody on the plate. After incubation and washing, the amount of cAMP-HRP bound to the plate can easily be determined by reading HRP activity at OD450 nm. The intensity of OD450 nm is inversely proportional to the concentration of cAMP in samples. In addition, the kit provides a new acetylation procedure to improve detection sensitivity significantly. The kit can detect ~0.1-10 pmol/5 µl (or ~ 0.02-2 µM) cAMP samples.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Adenosine 3’, 5’ cyclic monophosphate (cAMP) is an important second messenger in intracellular signal transduction. Monitoring cAMP levels is one of the most common ways to screen for agonists and antagonists of GPCRs.

     

    If you are using fluorometric detection, we recommend cAMP Direct Immunoassay Detection Kit (Fluorometric) (ab138880).

     

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Adenosine 3’,5’-cyclic monophosphate (cyclic AMP, cAMP) is an important “second messenger” involved in many physiological processes. It plays a key role as an intracellular second messenger for transduction events that follow a number of extracellular signals.
  • 别名
    • Adenosine 3’ 5’ cyclic monophosphate
    • Cyclic adenosine monophosphate
    • Cyclic AMP

应用

Our Abpromise guarantee covers the use of ab65355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

cAMP Direct Immunoassay Kit 图像

  • Measurement of cAMP in Jurkat and HepG2 cells with ab65355. Assay performed using the kit protocol.

  • Standard curve (colorimetric): mean of duplicates (+/-SD)

实验方案

cAMP Direct Immunoassay Kit (ab65355)参考文献

This product has been referenced in:
  • Ohyama K  et al. A combination of exercise and capsinoid supplementation additively suppresses diet-induced obesity by increasing energy expenditure in mice. Am J Physiol Endocrinol Metab 308:E315-23 (2015). Read more (PubMed: 25516550) »
  • Aspiras AC  et al. Melanocortin 4 receptor mutations contribute to the adaptation of cavefish to nutrient-poor conditions. Proc Natl Acad Sci U S A 112:9668-73 (2015). Read more (PubMed: 26170297) »

See all 12 Publications for this product

Product Wall

Thank you for contacting us regarding this protocol.

You are correct. There is no need to add a blocking step to this protocol. Further you do not need to wash the plates prior to adding the antibody or cAMP-HRP.

I hope that this info...

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I contacted the laboratories and they suggest that most labs generally have a bench-top vacuum centrifuge which I think would be ideal for this purpose, but if not, the oven drying should work as well. Use an oven at ˜60oC.

I


As this kit will detect a cyclic nucleotide and not a protein, it does not have a species restriction and should be fine for the use with bacteria.
However, we have not tested this ourselves, and therefore, cannot send you a protocol accordin...

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The species is not applicable for this kit. The species reactivity information we have on the datasheet is to show the kit was tested with human samples.

cAMP is a nucleotide which should be same in all species so this kit should work for thi...

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Thank you for your enquiry.

I am pleased to provide the suggested protocol for preparing cell lysate samples for this assay:

Cell Samples:

Aspirate medium.
Add 1 ml of 0.1M HCl for every 35 cm2 of surface area. Read More

Thank you for your reply.

It is difficult to know what happened with the kit without extra protocol and sample information. Perhaps you can find some helpful information in our ELISA resources pages:

http://www.abcam.com/index.html?...

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Thank you for contacting us.





In regards to your question as to whether ourcAMP Direct Immunoassay Kit, ab65355, will work for your need, yesthis assay can work beautifully for such a purpose. Please use all appropriat...

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Thank you for contacting us.

cAMP Direct Immunoassay Kit utilizes recombinant Protein G coated 96-well plate to anchor cAMP polyclonal antibody on to the plate.

I have read through the protocol and couldn’t find the need for a...

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Thank you for contacting us.

The standard concentration is indeed 2 pm/ul when diluted with 800ul of 0.1M HCL.

Step d (page 9) add 200ul of standard into 10pmol/50ul labelled vial, then we recommend doing the serial dilution. We t...

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Thank you for contacting us.

Although we have not tested these kits in plant samples ourselves, I do not foresee any problems with such use. However the protocols may have to be standardized/modified slightly by the end user.

Please...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"