概述

  • 产品名称
  • 描述
    羊多克隆抗体to cAMP
  • 宿主
    Sheep
  • 特异性
    This antibody reacts with cyclic AMP and to lesser degrees with other 5’ nucleotides.
  • 经测试应用
    适用于: ELISA, RIA, Othermore details
  • 种属反应性
    与反应: Species independent
  • 免疫原

    Succinylated cAMP conjugated to bovine serum albumin.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    serum with 0.01% sodium azide
  • 纯度
    Whole antiserum
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
AP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
This antibody may be used in radioimmunoassays to detect and quantitate cAMP; 50% binding is achieved with a 1:1,000 dilution.

 

Other Use at an assay dependent concentration.

靶标

  • 相关性
    Cyclic adenosine monophosphate (cAMP) plays a key role as an intracellular second messenger for transduction events that follow a number of extracellular signals. The G-Protein Coupled Receptors (GPCR) is the largest family of cell surface receptors. They can be activated by different ligands, such as neurotransmitters, hormones, ions, small molecules, peptides, and other physiological signaling molecules. Typically, the binding of the ligands to its receptor resulting in the activation of G-proteins, in return, activates the effector adenylyl cyclase evoking the production of cAMP. The activation of a protein kinase by cAMP results in the phosphorylation of substrate proteins. Currently successful drugs in marketing have been developed to target these receptors. Among the GPCRs, ~367 receptors are potential drug development targets, but only about 20 have been used to generate therapeutically and commercially successful drugs so far. Because the involvement of cAMP can amplify the response of the ligand binding, the second messenger cAMP has been largely employed to monitor the activation of the GPCR to facilitate the therapeutic drug discovery.
  • 细胞定位
    Secreted
  • 别名
    • 3' 5' cyclic adenosine monophosphate antibody
    • Cyclic adenosine monophosphate antibody
    • Cyclic AMP antibody

文献

This product has been referenced in:
  • Sweeny L  et al. Evaluation of tyrosine receptor kinases in the interactions of head and neck squamous cell carcinoma cells and fibroblasts. Oral Oncol 48:1242-9 (2012). Read more (PubMed: 22795534) »
  • Parro V  et al. A microbial oasis in the hypersaline Atacama subsurface discovered by a life detector chip: implications for the search for life on Mars. Astrobiology 11:969-96 (2011). Read more (PubMed: 22149750) »

See all 2 Publications for this product

客户评价及客户问答

ab832 may cross-react with other 5'-nucleotides, however sufficient dilution can eliminate this cross-reactivity while retaining reactivity with cAMP. Working dilution should be optimized by the end user.

Thank you for your enquiry. I have received replies from the four product originators. Here is the information that you requested for: ab12414 - First lots were sold in 1987 so before 2003. ab832 - Definitely before 2003. ab2345 - Not cert...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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