概述

  • 产品名称
  • 描述
    兔多克隆抗体to CAMKV
  • 经测试应用
    适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from an internal sequence of human CAMKV.

  • 阳性对照
    • Extracts from HepG2 and HUVEC cells. HeLa cells. Human Brain tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.2
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab69564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
ELISA 1/5000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能
    Does not appear to have detectable kinase activity.
  • 序列相似性
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • 结构域
    The protein kinase domain is predicted to be catalytically inactive.
  • 细胞定位
    Cell membrane. Cytoplasmic vesicle membrane. Predominantly observed in association with the plasma membrane of soma and in neurites, both axons and dendrites. May be associated with vesicular structures (By similarity).
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1G5 antibody
    • CaM kinase like vesicle associated antibody
    • CaM kinase like vesicle associated protein antibody
    • CaM kinase-like vesicle-associated protein antibody
    • camkv antibody
    • CAMKV_HUMAN antibody
    • MGC8407 antibody
    • OTTHUMP00000212745 antibody
    • OTTHUMP00000212766 antibody
    • VACAMKL antibody
    • Vesicle associated calmodulin binding protein antibody
    see all

图片

  • All lanes : Anti-CAMKV antibody (ab69564) at 1/500 dilution

    Lane 1 : HepG2 cells extracts
    Lane 2 : HUVEC cells extracts
    Lane 3 : HepG2 cells extracts with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 54 kDa
    Observed band size : 54 kDa
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using 1/50 ab69564. For the image on the right, the antibody was pre-incubated with the immunizing peptide.
  • Immunofluorescence analysis of HeLa cells, using 1/500 ab69564. For the image on the right, the antibody was pre-incubated with the immunizing peptide.

文献

This product has been referenced in:
  • O'Connell DJ  et al. Integrated protein array screening and high throughput validation of 70 novel neural calmodulin binding proteins. Mol Cell Proteomics : (2010). WB ; Mouse . Read more (PubMed: 20068228) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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