Anti-CAMKK2抗体- C-terminal (ab70909)

概述

  • 产品名称Anti-CAMKK2抗体- C-terminal
    参阅全部 CAMKK2 一抗
  • 描述
    兔多克隆抗体to CAMKK2 - C-terminal
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide from the C-terminal region of human CAMKK2 conjugated to KLH.

  • 阳性对照
    • 293 cell lysates transiently transfected with the CAMKK2 gene. Human brain tissue.

性能

应用

Our Abpromise guarantee covers the use of ab70909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50 - 1/100. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
ELISA 1/1000.
IHC-P 1/50.

靶标

  • 功能Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Seems to be involved in hippocampal activation of CREB1.
  • 组织特异性Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung.
  • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • 结构域The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.
    The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates.
  • 翻译后修饰Autophosphorylated.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Calcium/calmodulin dependent protein kinase beta antibody
    • Calcium/calmodulin dependent protein kinase kinase 2 antibody
    • Calcium/calmodulin dependent protein kinase kinase 2 beta antibody
    • Calcium/calmodulin dependent protein kinase kinase beta antibody
    • Calcium/calmodulin-dependent protein kinase kinase 2 antibody
    • Calcium/calmodulin-dependent protein kinase kinase beta antibody
    • CaM kinase kinase beta antibody
    • CaM KK beta antibody
    • CaM-kinase kinase 2 antibody
    • CaM-kinase kinase beta antibody
    • CaM-KK 2 antibody
    • CaM-KK beta antibody
    • CaMKK 2 antibody
    • CAMKK antibody
    • CaMKK beta antibody
    • CAMKK beta protein antibody
    • Camkk2 antibody
    • CAMKKB antibody
    • KKCC2_HUMAN antibody
    see all

Anti-CAMKK2 antibody - C-terminal 图像

  • All lanes : Anti-CAMKK2 antibody - C-terminal (ab70909) at 1/50 dilution

    Lane 1 : Nontransfected 293 cell lysate
    Lane 2 : 293 cell lysate transiently transfected with the CAMKK2 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 65 kDa
    Observed band size : 65 kDa
    Additional bands at : 62 kDa. We are unsure as to the identity of these extra bands.
  • ab70909, at 1/50 dilution, staining CAMKK2 in human brain tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue followed by a peroxidase-conjugated secondary antibody and DAB staining.

Anti-CAMKK2 antibody - C-terminal (ab70909)参考文献

ab70909 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (spinal cord)
Specification spinal cord
Fixative Paraformaldehyde
Permeabilization No
Username

Dr. Karine Thibault

Verified customer

提交于 Dec 14 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"