Anti-CAMKIV (phospho T196 + T200)抗体(ab59424)

概述

  • 产品名称Anti-CAMKIV (phospho T196 + T200)抗体
    参阅全部 CAMKIV 一抗
  • 描述
    兔多克隆抗体to CAMKIV (phospho T196 + T200)
  • 特异性Detects endogenous levels of CaMKIV only when phosphorylated at threonine 196 and 200
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from human CAMKIV around the phosphorylation site of threonine 196/200 (M-K-TP-V-C).

  • 阳性对照
    • Brain tissue

性能

应用

Our Abpromise guarantee covers the use of ab59424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 52 kDa. PubMed: 23593332
ELISA 1/40000.
IHC-P 1/50 - 1/100.

靶标

  • 功能Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory.
  • 组织特异性Expressed in epithelial ovarian cancer tissue.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200.
  • 细胞定位Cytoplasm. Nucleus. Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody
    • Brain Ca(2+) calmodulin dependent protein kinase type IV antibody
    • Brain Ca++-calmodulin dependent protein kinase type IV antibody
    • Calcium / calmodulin dependent protein kinase type 4 catalytic chain antibody
    • Calcium / calmodulin dependent protein kinase type IV catalytic chain antibody
    • Calcium/calmodulin dependent protein kinase IV antibody
    • Calcium/calmodulin dependent protein kinase type IV antibody
    • Calcium/calmodulin-dependent protein kinase type IV antibody
    • CAM kinase 4 antibody
    • CAM kinase GR antibody
    • CAM kinase IV antibody
    • CAM kinase-GR antibody
    • CaMK 4 antibody
    • CAMK GR antibody
    • CaMK IV antibody
    • Camk4 antibody
    • CaMKGR antibody
    • IV antibody
    • KCC4_HUMAN antibody
    • MGC36771 antibody
    see all

Anti-CAMKIV (phospho T196 + T200) antibody 图像

  • ab59424, at a 1/50 dilution, staining paraffin embedded human brain tissue sections by Immunohistochemistry in the absence (left image) or presence (right image) of the phosphopeptide.

Anti-CAMKIV (phospho T196 + T200) antibody (ab59424)参考文献

This product has been referenced in:
  • Moriguchi S  et al. Stimulation of the sigma-1 receptor by DHEA enhances synaptic efficacy and neurogenesis in the hippocampal dentate gyrus of olfactory bulbectomized mice. PLoS One 8:e60863 (2013). WB . Read more (PubMed: 23593332) »
  • Oury F  et al. CREB mediates brain serotonin regulation of bone mass through its expression in ventromedial hypothalamic neurons. Genes Dev 24:2330-42 (2010). WB ; Mouse . Read more (PubMed: 20952540) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Zebrafish Tissue lysate - whole (zf embryo)
Loading amount 30 µg
Specification zf embryo
Treatment de-yolked
Gel Running Conditions Non-reduced Denaturing (10% gel)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Ms. Gemma Lace

Verified customer

提交于 Jan 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"