概述

  • 产品名称
    Anti-CAMKIV抗体[EP2564Y]
    参阅全部 CAMKIV 一抗
  • 描述
    兔单克隆抗体[EP2564Y] to CAMKIV
  • 经测试应用
    适用于: WB, ICCmore details
    不适用于: Flow Cyt,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide from the N terminal region of human CaMKIV.

  • 阳性对照
    • Jurkat cell lysate and human brain lysate.
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab75874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Predicted molecular weight: 52 kDa.
ICC 1/100 - 1/250.
  • 应用说明
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • 靶标

    • 功能
      Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory.
    • 组织特异性
      Expressed in epithelial ovarian cancer tissue.
    • 序列相似性
      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
      Contains 1 protein kinase domain.
    • 翻译后修饰
      Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200.
    • 细胞定位
      Cytoplasm. Nucleus. Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody
      • Brain Ca(2+) calmodulin dependent protein kinase type IV antibody
      • Brain Ca++-calmodulin dependent protein kinase type IV antibody
      • Calcium / calmodulin dependent protein kinase type 4 catalytic chain antibody
      • Calcium / calmodulin dependent protein kinase type IV catalytic chain antibody
      • Calcium/calmodulin dependent protein kinase IV antibody
      • Calcium/calmodulin dependent protein kinase type IV antibody
      • Calcium/calmodulin-dependent protein kinase type IV antibody
      • CAM kinase 4 antibody
      • CAM kinase GR antibody
      • CAM kinase IV antibody
      • CAM kinase-GR antibody
      • CaMK 4 antibody
      • CAMK GR antibody
      • CaMK IV antibody
      • Camk4 antibody
      • CaMKGR antibody
      • IV antibody
      • KCC4_HUMAN antibody
      • MGC36771 antibody
      see all

    图片

    • All lanes : Anti-CAMKIV antibody [EP2564Y] (ab75874) at 1/2000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Human brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size : 52 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)

    文献

    This product has been referenced in:
    • Yan X  et al. CaMKII-Mediated CREB Phosphorylation Is Involved in Ca2+-Induced BDNF mRNA Transcription and Neurite Outgrowth Promoted by Electrical Stimulation. PLoS One 11:e0162784 (2016). WB, ICC/IF ; Rat . Read more (PubMed: 27611779) »

    See 1 Publication for this product

    客户评价及客户问答

    Thank you for your reply.
    Reviewing the remaining antibodies that are already tested in WB and the species you wish to use, the following do not yet have a western blot image, and I would be pleased to arrange a testing discount for these addition...

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    Thank you very much for your interest in our antibodies.

    We appreciate receiving feedback and data from customers, and we do have a testing discount offer available.

    If a customer would like to test an antibody in an application o...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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