The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000 - 1/15000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
Use at 3-5 µg/mg of lysate.
1/200 - 1/1000.
功能Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory.
组织特异性Expressed in epithelial ovarian cancer tissue.
序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain.
翻译后修饰Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200.
细胞定位Cytoplasm. Nucleus. Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix.
Lane3: 250µg Jurkat whole cell lysate immunoprecipitated with ab70229 at 3 µg/mg lysate
Lane4: 250µg Jurkat whole cell lysate with IgG control.
All lanes blotted with ab70229 at 0.04 µg/ml.
Lane 1 and Lane 2: samples immunoprecipitated with irrelevant antibodies.
Anti-CAMKIV antibody (ab70229)参考文献
has not yet been referenced specifically in any publications.