概述

  • 产品名称Anti-CAMKIV抗体
    参阅全部 CAMKIV 一抗
  • 描述
    兔多克隆抗体to CAMKIV
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region between residue 425 and the C-terminus (residue 473) of human CAMKIV (Swiss-Prot Q16566).

  • 阳性对照
    • Whole cell lysate from Jurkat and HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab70229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/15000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
IP Use at 3-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000.

靶标

  • 功能Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory.
  • 组织特异性Expressed in epithelial ovarian cancer tissue.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200.
  • 细胞定位Cytoplasm. Nucleus. Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody
    • Brain Ca(2+) calmodulin dependent protein kinase type IV antibody
    • Brain Ca++-calmodulin dependent protein kinase type IV antibody
    • Calcium / calmodulin dependent protein kinase type 4 catalytic chain antibody
    • Calcium / calmodulin dependent protein kinase type IV catalytic chain antibody
    • Calcium/calmodulin dependent protein kinase IV antibody
    • Calcium/calmodulin dependent protein kinase type IV antibody
    • Calcium/calmodulin-dependent protein kinase type IV antibody
    • CAM kinase 4 antibody
    • CAM kinase GR antibody
    • CAM kinase IV antibody
    • CAM kinase-GR antibody
    • CaMK 4 antibody
    • CAMK GR antibody
    • CaMK IV antibody
    • Camk4 antibody
    • CaMKGR antibody
    • IV antibody
    • KCC4_HUMAN antibody
    • MGC36771 antibody
    see all

Anti-CAMKIV antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling CAMKIV with ab70229 at 1/1000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-CAMKIV antibody (ab70229) at 0.04 µg/ml

    Lane 1 : Jurkat whole cell lysate at 50 µg
    Lane 2 : Jurkat whole cell lysate at 15 µg
    Lane 3 : Jurkat whole cell lysate at 5 µg
    Lane 4 : HeLa whole cell lysate at 50 µg


    Predicted band size : 52 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • Lane3: 250µg Jurkat whole cell lysate immunoprecipitated with ab70229 at 3 µg/mg lysate Lane4: 250µg Jurkat whole cell lysate with IgG control. All lanes blotted with ab70229 at 0.04 µg/ml. Lane 1 and Lane 2: samples immunoprecipitated with irrelevant antibodies.

Anti-CAMKIV antibody (ab70229)参考文献

ab70229 has not yet been referenced specifically in any publications.

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