Anti-CaMKII (phospho T286)抗体[22B1] (ab2724)


  • 产品名称Anti-CaMKII (phospho T286)抗体[22B1]
    参阅全部 CaMKII 一抗
  • 描述
    小鼠单克隆抗体[22B1] to CaMKII (phospho T286)
  • 特异性Detects phosphorylated CaMKII from rat tissues. This antibody is specific for a and ß subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in ß).
  • 经测试应用适用于: Flow Cyt, ICC/IF, ELISA, IP, WBmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Rat CaMKII aa 281-294.
    Database link: Q9UQM7

  • 阳性对照
    • WB: rat brain homogenate ICC: rat hippocampal cells in culture
  • 常规说明

    CaM kinase II, in a calcium and calmodulin dependent manner, phosphorylates many different brain substrates including synapsin I, tryptophan hydroxylase, tyrosine hydroxylase and nitric oxide synthase thereby performing regulatory functions associated with increases in intracellular free calcium. CaM kinase II is particularly abundant in the hippocampus and forebrain where it comprises ~1% of total protein. Within the neuron, CaM kinase II is a major component of the postsynaptic density fraction accounting for as much as 30% of total protein in the post synaptic density (PSD) region. Autophosphorylation of threonine-286 occurs after calcium/calmodulin activation and can enable CaM kinase II to remain active independent of free calcium. Autophosphorylation of CaM kinase II in hippocampal neurons, which results in relatively high levels of kinase activity even at basal calcium concentrations, may allow for both upward and downward regulation as opposed to simply on/off regulation.



Our Abpromise guarantee covers the use of ab2724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells.
ICC/IF 1/100.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).


Anti-CaMKII (phospho T286) antibody [22B1] 图像

  • Anti-CaMKII (phospho T286) antibody [22B1] (ab2724) at 1/1000 dilution + Mouse Hippocampal whole tissue lysate. at 20 µg

    HRP conjugated rabbit anti-mouse antibody. at 1/2500 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 54 kDa

    Exposure time : 10 seconds

    This image is courtesy of an anonymous abreview.

    The blot was blocked with 5% BSA for 1 hour at room temperature.

    See Abreview

  • Predicted band size : 54 kDa

    Lane 1: unphosphorylated rat brain homogenate - ab2724
    Lane 2: phosphorylated rat brain homogenate - ab2724
    Lane 3: unphosphorylated rat brain homogenate - ab2725
    Lane 4: phosphorylated rat brain homogenate - ab2725

  • ICC/IF image of ab2724 stained Hep cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2724, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing SH-SY5Y cells stained with ab2724 (red line). The cells were fixed with 4% paraformaldehyde and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2724, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-CaMKII (phospho T286) antibody [22B1] (ab2724)参考文献

This product has been referenced in:
  • Jarome TJ  et al. CaMKII, but not protein kinase A, regulates Rpt6 phosphorylation and proteasome activity during the formation of long-term memories. Front Behav Neurosci 7:115 (2013). WB ; Rat . Read more (PubMed: 24009566) »
  • Jung SC  et al. Rapid, bidirectional remodeling of synaptic NMDA receptor subunit composition by A-type K+ channel activity in hippocampal CA1 pyramidal neurons. Neuron 60:657-71 (2008). ICC/IF ; Rat . Read more (PubMed: 19038222) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Hippocampus)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 20 µg
Specification Hippocampus
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

提交于 Nov 24 2014

The testing has revealed that the mouse clone 22B1 is actually producing IgG1 kappa, so you should be fine to use your chosen secondary antibody.

Thank you for your inquiry.
I am sorry to confirm that despite this clone having been around for more than 20 years the light chains have never been characterized.
The lab will test this, but I have to confirm that this will take some time an...

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Thank you for contacting us. Sandwich ELISA procedures can be difficult to optimize and tested match pair antibodies should be used. This ensures the antibodies are detecting different epitopes on the target protein so they do not interfere wit...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Sections of rat spinal cord and brain)
Specification Sections of rat spinal cord and brain
Fixative Paraformaldehyde

Dr. Sophie Pezet

Verified customer

提交于 Dec 28 2005

Thank you for your enquiry and your interest in our products. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replace...

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