概述

  • 产品名称
    Anti-CaMKII beta抗体
    参阅全部 CaMKII beta 一抗
  • 描述
    兔多克隆抗体to CaMKII beta
  • 特异性
    This antibody does cross-react with CAMKII alpha protein
  • 经测试应用
    适用于: IHC-FoFr, ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Mouse CaMKII beta.

    (Peptide available as ab27843.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Brain (Mouse) Tissue Brain (Rat) Tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: PC12.

性能

应用

Our Abpromise guarantee covers the use of ab34703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr 1/300.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).Can be blocked with Mouse CaMKII beta peptide (ab27843).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

靶标

  • 功能
    CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
  • 组织特异性
    Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain.
  • 序列相似性
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • 数据库链接
  • 形式
    The multifunctional CaMKII beta, or Ca2+/calmodulin-dependent protein kinase II, is a well known effector of calcium- and calmodulin- mediated functions. It is present in many tissues but is most abundant in the brain. CaMKII is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits.
  • 别名
    • Calcium/calmodulin dependent protein kinase (CaM kinase) II beta antibody
    • Calcium/calmodulin dependent protein kinase II beta antibody
    • Calcium/calmodulin dependent protein kinase IIB antibody
    • Calcium/calmodulin dependent protein kinase type II beta chain antibody
    • calcium/calmodulin-dependent protein kinase II beta antibody
    • Calcium/calmodulin-dependent protein kinase type II subunit beta antibody
    • CAM 2 antibody
    • CaM kinase II beta chain antibody
    • CaM kinase II beta subunit antibody
    • CaM kinase II subunit beta antibody
    • CaM-kinase II beta chain antibody
    • CAM2 antibody
    • CAMK 2 antibody
    • CAMK 2B antibody
    • CaMK II beta subunit antibody
    • CaMK II subunit beta antibody
    • CaMK-II subunit beta antibody
    • CAMK2 antibody
    • CaMK2 beta antibody
    • Camk2b antibody
    • CAMKB antibody
    • CaMKII beta subunit antibody
    • CaMKIIB antibody
    • KCC2B_HUMAN antibody
    • MGC29528 antibody
    • Proline rich calmodulin dependent protein kinase antibody
    • proline rich calmodulin-dependent protein kinase antibody
    see all

Anti-CaMKII beta antibody 图像

  • All lanes : Anti-CaMKII beta antibody (ab34703) at 1 µg/ml

    Lane 1 : Brain Mouse Tissue Lysate
    Lane 2 : Brain Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
  • ab34703 staining CaMKII in rat brain tissue section (hippocampus) by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/300 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo488 conjugated goat polyclonal to rabbit IgGwas used as secondary at 1/1000 dilution.

    See Abreview

  • ab34703 staining CaMKII beta in adult mouse cerebellum tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using 0.01M Tris HCL, pH 10. Samples were then permeabilized using 0.3% Triton X-100, blocked with 5% BSA for 45 minutes at 22°C and then incubated with ab34703 at a 1/3000 dilution for 16 hours at 4°C. The secondary used was a biotin conjugated horse polyclonal used at a 1/2000 dilution.

    Strong siganls were detected in molecular layers (M), and Purkinje cells (P) were positively stained as well. In granule layers (G), some neurons were also strongly stained.

    See Abreview

  • ICC/IF image of ab34703 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab34703 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CaMKII beta antibody (ab34703)参考文献

This product has been referenced in:
  • Li Y  et al. Role of the Lateral Habenula in Pain-Associated Depression. Front Behav Neurosci 11:31 (2017). WB . Read more (PubMed: 28270756) »
  • Kool MJ  et al. The molecular, temporal and region-specific requirements of the beta isoform of Calcium/Calmodulin-dependent protein kinase type 2 (CAMK2B) in mouse locomotion. Sci Rep 6:26989 (2016). Read more (PubMed: 27244486) »

See all 11 Publications for this product

Product Wall

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Pancreatic Cells)
Gel Running Conditions
Reduced Denaturing (4-12% gel)
Loading amount
20 µg
Specification
Pancreatic Cells
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 01 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Specification
Pancreas
Blocking step
Casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 31 2017

Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Non-reduced Denaturing
Sample
Rat Tissue lysate - whole (hippocampus tissue lysate)
Specification
hippocampus tissue lysate
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Username

Abcam user community

Verified customer

提交于 Jun 20 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse whole brain)
Loading amount
10 µg
Specification
mouse whole brain
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Dec 02 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse cerebellum)
Specification
mouse cerebellum
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01 M Tris HCL, pH 10
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Dec 02 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

提交于 Mar 17 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

提交于 Mar 17 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册