概述

  • 产品名称Anti-CaMKII beta抗体
    参阅全部 CaMKII beta 一抗
  • 描述
    兔多克隆抗体to CaMKII beta
  • 特异性This antibody does cross-react with CAMKII alpha protein
  • 经测试应用适用于: ELISA, WB, IHC (PFA fixed)more details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    VRRGSGTPEAEAPRQ

    , corresponding to C terminal amino acids 499-513 of Human CaMKII beta

  • 阳性对照
    • Human, mouse or rat brain.

应用

Our Abpromise guarantee covers the use of ab22131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
IHC (PFA fixed)
  • 应用说明ELISA: 1/100 - 1/1000.
    Immunohistochemistry (PFA fixed): 1/100.
    WB: 1/1000 - 1/5000. Detects a band of approximately 67 kDa (predicted molecular weight: 73 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
    • 组织特异性Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain.
    • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
      Contains 1 protein kinase domain.
    • Information by UniProt
    • 数据库链接
    • 形式The multifunctional CaMKII beta, or Ca2+/calmodulin-dependent protein kinase II, is a well known effector of calcium- and calmodulin- mediated functions. It is present in many tissues but is most abundant in the brain. CaMKII is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits.
    • 别名
      • Calcium/calmodulin dependent protein kinase (CaM kinase) II beta antibody
      • Calcium/calmodulin dependent protein kinase II beta antibody
      • Calcium/calmodulin dependent protein kinase IIB antibody
      • Calcium/calmodulin dependent protein kinase type II beta chain antibody
      • calcium/calmodulin-dependent protein kinase II beta antibody
      • Calcium/calmodulin-dependent protein kinase type II subunit beta antibody
      • CAM 2 antibody
      • CaM kinase II beta chain antibody
      • CaM kinase II beta subunit antibody
      • CaM kinase II subunit beta antibody
      • CaM-kinase II beta chain antibody
      • CAM2 antibody
      • CAMK 2 antibody
      • CAMK 2B antibody
      • CaMK II beta subunit antibody
      • CaMK II subunit beta antibody
      • CaMK-II subunit beta antibody
      • CAMK2 antibody
      • CaMK2 beta antibody
      • Camk2b antibody
      • CAMKB antibody
      • CaMKII beta subunit antibody
      • CaMKIIB antibody
      • KCC2B_HUMAN antibody
      • MGC29528 antibody
      • Proline rich calmodulin dependent protein kinase antibody
      • proline rich calmodulin-dependent protein kinase antibody
      see all

    Anti-CaMKII beta antibody 图像

    • Immunofluorescent staining in the rat hippocampal neurons using ab22131 CAMKIIb antibody. This picture was taken using a 40X objective. The staining is observed in the axons and cell bodies of some hippocampal neurons. NB: apart from the hippocampus, most brain areas did not show any staining in this naïve animal. ab22131 was used at 1/100 incubated for 2 days at room temperature. Secondary antibody used was anti-rabbit Alexa Fluor 488 at 1/1000 incubated for 2 hours at room temperature. Rat brain tissue was perfusion fixed with 4% PFA followed by overnight post-fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut on a cyrostat and immunohistochemistry performed by the 'free floating' technique.

    • Anti-CaMKII beta antibody (ab22131) at 1/2000 dilution + Rat brain lysate

      Predicted band size : 73 kDa
      Observed band size : 67 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-CaMKII beta antibody (ab22131) at 1/2000 dilution

      Lane 1 : Human brain lysate
      Lane 2 : Mouse brain lysate
      Lane 3 : Rat brain lysate


      Predicted band size : 73 kDa

    Anti-CaMKII beta antibody (ab22131)参考文献

    This product has been referenced in:
    • Han MH  et al. Proteomics analysis reveals overlapping functions of clustered protocadherins. Mol Cell Proteomics 9:71-83 (2010). Read more (PubMed: 19843561) »
    • Fazi B  et al. Characterization of gene expression induced by RTN-1C in human neuroblastoma cells and in mouse brain. Neurobiol Dis 40:634-44 (2010). WB ; Mouse . Read more (PubMed: 20708685) »

    See all 2 Publications for this product

    Product Wall

    Vielen Dank für Ihre Anfrage.

    Leider ist es nicht möglich die Konzentration von ab22131 zu bestimmen.

    Bei ab22131 handelt es sich um unaufgereinigtes Vollserum.

    Die Konzentration von Antikörpern wird üb...

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    Vielen Dank für Ihre Anfrage.

    Ich kann empfehlen den ab87597 mit einer Verdünnungreihe, ausgehend von 1/100 )zum Beispiel 1/50, 1/100 und 1/200) auszutested. Auch kann ich empfehlen, die Antigendemaskierung mit einem Citrate Puffer pH 6 unte...

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    Thank you for contacting us with your question. For a whole rabbit serum antibody like ab22131, we estimate that the total IgG concentration is 12-15 mg/mL. The total IgG population will contain both the specific CaMKII beta antibodies and other ant...

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    Thank you for your enquiry. I have attached a JPeG image of this antibody applied by western blotting in a follow up email. The band appears just above the 66 kDa marker. However, CAMKII beta can be expressed as different isoforms. Please follo...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"