重组Anti-CaMKII抗体[EP1829Y] (ab52476)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1829Y] to CaMKII
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-CaMKII抗体[EP1829Y]
参阅全部 CaMKII 一抗 -
描述
兔单克隆抗体[EP1829Y] to CaMKII -
宿主
Rabbit -
特异性
The peptide immunogen is highly conserved between CaMKII alpha, beta, gamma, and delta. -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human CaMKII aa 200-300. The exact sequence is proprietary.
(Peptide available asab199551) -
阳性对照
- WB: Mouse brain, rat brain and human fetal brain tisse lysates. IHC-P: Human hepatocellulas carcinoma tissue. ICC/IF: U87-MG and PC12 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1829Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- HRP Anti-CaMKII antibody [EP1829Y] (ab195518)
- Alexa Fluor® 647 Anti-CaMKII antibody [EP1829Y] (ab196165)
- Alexa Fluor® 488 Anti-CaMKII antibody [EP1829Y] (ab196378)
- Alexa Fluor® 555 Anti-CaMKII antibody [EP1829Y] (ab206883)
- Anti-CaMKII antibody [EP1829Y] - Low endotoxin, Azide free (ab219365)
- Anti-CaMKII antibody [EP1829Y] - BSA and Azide free (ab271841)
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52476于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/250.
|
|
WB | (7) |
1/1000 - 1/5000. Detects a band of approximately 45, 70 kDa (predicted molecular weight: 54 kDa).
For unpurified use at 1/20000. |
IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
说明 |
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ICC/IF
1/250. |
WB
1/1000 - 1/5000. Detects a band of approximately 45, 70 kDa (predicted molecular weight: 54 kDa). For unpurified use at 1/20000. |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
细胞定位
Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts. - Information by UniProt
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数据库链接
- Entrez Gene: 815 Human
- Entrez Gene: 816 Human
- Entrez Gene: 817 Human
- Entrez Gene: 818 Human
- Entrez Gene: 108058 Mouse
- Entrez Gene: 12322 Mouse
- Entrez Gene: 12323 Mouse
- Entrez Gene: 12325 Mouse
see all -
别名
- Calcium/calmodulin dependent protein kinase II alpha antibody
- Calcium/calmodulin dependent protein kinase II beta antibody
- Calcium/calmodulin dependent protein kinase II delta antibody
see all
图片
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Anti-CaMKII antibody [EP1829Y] (ab52476) at 1/5000 dilution (purified) + Human fetal brain tissue lysate at 20 µg
Secondary
HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 45,70 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-CaMKII antibody [EP1829Y] (ab52476) at 1/1000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 45,70 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Anti-CaMKII antibody [EP1829Y] (ab52476) at 1/20000 dilution (unpurified) + Mouse brain lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 54 kDa
Observed band size: 45,70 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling CaMKII with purified ab52476 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunocytochemistry/Immunofluorescence analysis of U87-MG cells labelling CaMKII with purified ab52476 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% aTriton X-100. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
Control: primary antibody (1/250) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).
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ICC/IF image of unpurified ab52476 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52476, 1µg/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (99)
ab52476 被引用在 99 文献中.
- Zhang XZ et al. Insulin-like growth factor-1 inhibits apoptosis of rat gastric smooth muscle cells under high glucose condition via adenosine monophosphate-activated protein kinase (AMPK) pathway. Folia Histochem Cytobiol 60:74-88 (2022). PubMed: 35156189
- Wei B et al. T1AM Attenuates the Hypoxia/Reoxygenation-Induced Necroptosis of H9C2 Cardiomyocytes via RIPK1/RIPK3 Pathway. Biomed Res Int 2022:4833791 (2022). PubMed: 35265713
- Zhang Y et al. Follistatin-like 1 (FSTL1) interacts with Wnt ligands and Frizzled receptors to enhance Wnt/β-catenin signaling in obstructed kidneys in vivo. J Biol Chem 298:102010 (2022). PubMed: 35525270
- Yu J et al. Effects and potential mechanism of Ca2+/calmodulin‑dependent protein kinase II pathway inhibitor KN93 on the development of ovarian follicle. Int J Mol Med 50:N/A (2022). PubMed: 35929517
- Mi S et al. CaMKII is a modulator in neurodegenerative diseases and mediates the effect of androgen on synaptic protein PSD95. Front Genet 13:959360 (2022). PubMed: 35991539