Anti-CaMKII抗体[6G9] (ab22609)

概述

  • 产品名称
    Anti-CaMKII抗体[6G9]
    参阅全部 CaMKII 一抗
  • 描述
    小鼠单克隆抗体[6G9] to CaMKII
  • 特异性
    This antibody detects the CaMKII alpha subunit in both phosphorylated and non phosphorylated forms. Expected reactivity also includes the delta and gamma isoforms but not the beta isoform.
  • 经测试应用
    适用于: IHC-Fr, IP, IHC-P, ICC/IF, WB, RIA, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow
  • 免疫原

    Partially purified full length native rat protein.

  • 阳性对照
    • WB: Human, rat and mouse whole brain tissue lysates. Flow Cyt: PC12 cells. IHC - P: Rat normal brain FFPE tissue sections.
  • 常规说明

    This antibody clone is manufactured by Abcam.

性能

应用

Our Abpromise guarantee covers the use of ab22609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 22201125
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).
RIA Use a concentration of 3 - 5 µg/ml.
ELISA Use at an assay dependent concentration.

靶标

图片

  • IHC image of ab22609 staining in Rat normal brain (Purkinje cells, cerebellum) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22609, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • All lanes : Anti-CaMKII antibody [6G9] (ab22609) at 10 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,65 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes

文献

This product has been referenced in:
  • Geng KW  et al. Ethanol Increases Mechanical Pain Sensitivity in Rats via Activation of GABAA Receptors in Medial Prefrontal Cortex. Neurosci Bull N/A:N/A (2016). IF . Read more (PubMed: 27628528) »
  • Tada H  et al. Neonatal isolation augments social dominance by altering actin dynamics in the medial prefrontal cortex. Proc Natl Acad Sci U S A N/A:N/A (2016). Read more (PubMed: 27791080) »

See all 22 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Frozen sections)
Sample
Medaka fish Tissue sections (brain)
Permeabilization
No
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 03 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Dorsal Raphe Nucleus)
Antigen retrieval step
None
Permeabilization
Yes - 0.3% Triton X-100
Specification
Dorsal Raphe Nucleus
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formalin
Username

Abcam user community

Verified customer

提交于 Oct 26 2015

Thank you for contacting us.

The Bovine species for all of these antibodies is predicted. I am sorry we do not hold any bovine specific data.

The immunogen of these antibodies are as follows;

- ab22609; Partially purified ful...

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Thank you for contacting us. Sandwich ELISA procedures can be difficult to optimize and tested match pair antibodies should be used. This ensures the antibodies are detecting different epitopes on the target protein so they do not interfere wit...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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