Anti-CaMKII alpha抗体[EPR1828] (ab92332)


  • 产品名称Anti-CaMKII alpha抗体[EPR1828]
    参阅全部 CaMKII alpha 一抗
  • 描述
    兔单克隆抗体[EPR1828] to CaMKII alpha
  • 特异性The peptide immunogen shares a 100% homology with CaMKII beta, gamma and delta therefore we believe cross-reactivity is highly likely but this has not been experimentally assessed.
  • 经测试应用适用于: WB, IP, IHC (PFA fixed), Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the N terminal of Human CaMKII alpha.

  • 阳性对照
    • Rat brain lysate, SH SY5Y cell lysate, Neuro 2a cell lysate
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab92332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Predicted molecular weight: 54 kDa.
IP 1/10 - 1/100.
IHC (PFA fixed) Use at an assay dependent concentration. PubMed: 24866701
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • 功能CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • 细胞定位Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha CaMKII antibody
    • Calcium calmodulin dependent protein kinase II antibody
    • Calcium/calmodulin dependent protein kinase II alpha B subunit antibody
    • Calcium/calmodulin dependent protein kinase type II alpha chain antibody
    • Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha antibody
    • Calcium/calmodulin-dependent protein kinase II alpha antibody
    • Calcium/calmodulin-dependent protein kinase II-alpha antibody
    • Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody
    • Calcium/calmodulin-dependent protein kinase type IIA antibody
    • CaM kinase II alpha chain antibody
    • CaM kinase II alpha subunit antibody
    • CaM kinase II subunit alpha antibody
    • CaMK II alpha subunit antibody
    • CaMK-II subunit alpha antibody
    • Camk2a antibody
    • CAMKA antibody
    • CaMKII antibody
    • CaMKIINalpha antibody
    • EC antibody
    • KCC2A_HUMAN antibody
    • KIAA0968 antibody
    • MGC123320 antibody
    • MGC139375 antibody
    • MGC155201 antibody
    • mKIAA0968 antibody
    • PK2CDD antibody
    • PKCCD antibody
    • R74975 antibody
    • zgc:112538 antibody
    • zgc:123320 antibody
    see all

Anti-CaMKII alpha antibody [EPR1828] 图像

  • Overlay histogram showing SH-SY5Y cells stained with ab92332 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92332, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • All lanes : Anti-CaMKII alpha antibody [EPR1828] (ab92332)

    Lane 1 : Rat brain lysate
    Lane 2 : SH SY5Y cell lysate
    Lane 3 : Neuro 2a cell lysate

    Lysates/proteins at 10 µg per lane.

    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 54 kDa

Anti-CaMKII alpha antibody [EPR1828] (ab92332)参考文献

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Thank you for contacting us. Sandwich ELISA procedures can be difficult to optimize and tested match pair antibodies should be used. This ensures the antibodies are detecting different epitopes on the target protein so they do not interfere wit...

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