Anti-CaMKII alpha抗体(ab111890)
Key features and details
- Goat polyclonal to CaMKII alpha
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-CaMKII alpha抗体
参阅全部 CaMKII alpha 一抗 -
描述
山羊多克隆抗体to CaMKII alpha -
宿主
Goat -
特异性
ab111890 is expected to recognize both reported isoforms (NP_057065.2; NP_741960.1). This antibody does cross-react with CAMKII beta protein -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Cow, Dog -
免疫原
Synthetic peptide, C-
PRTAQSEETRVWHR
, from the internal region of Human CaMKII alpha (NP_057065.2; NP_741960.1). -
阳性对照
- Human adrenal tissue and Human brain (Cortex) tissue Mouse brain lysate and Human Brain (Cerebellum) lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab111890于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 55, 65 kDa (predicted molecular weight: 54 kDa).
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IHC-P |
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 55, 65 kDa (predicted molecular weight: 54 kDa). |
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
细胞定位
Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts. - Information by UniProt
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数据库链接
- Entrez Gene: 530719 Cow
- Entrez Gene: 815 Human
- Entrez Gene: 12322 Mouse
- Entrez Gene: 25400 Rat
- Omim: 114078 Human
- SwissProt: Q9UQM7 Human
- SwissProt: P11798 Mouse
- SwissProt: P11275 Rat
see all -
别名
- Alpha CaMKII antibody
- Calcium calmodulin dependent protein kinase II antibody
- Calcium/calmodulin dependent protein kinase II alpha B subunit antibody
see all
图片
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Anti-CaMKII alpha antibody (ab111890) at 0.1 µg/ml + Mouse brain lysate in RIPA buffer at 35 µg
Predicted band size: 54 kDa
Primary incubation was 1 hour. Detected by chemiluminescence. -
ab111890, at 3.75 µg/ml, staining CaMKII alpha in formalin fixed, paraffin embedded Human adrenal by Immunohistochemistry.
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ab111890, at 3.75 µg/ml, staining CaMKII alpha in formalin fixed, paraffin embedded Human brain cortex by Immunohistochemistry.
数据表及文件
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Datasheet download
文献 (1)
ab111890 被引用在 1 文献中.
- Moore KM et al. Glutamate Afferents From the Medial Prefrontal Cortex Mediate Nucleus Accumbens Activation by Female Sexual Behavior. Front Behav Neurosci 13:227 (2019). PubMed: 31636548