概述

  • 产品名称Anti-CaMKI gamma抗体
    参阅全部 CaMKI gamma 一抗
  • 描述
    小鼠单克隆抗体to CaMKI gamma
  • 经测试应用适用于: WB, Indirect ELISAmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-477 of Human CaMKI gamma (AAH32787) with a 26kDa enzyme tag.

  • 阳性对照
    • Transfected lysate

性能

应用

Our Abpromise guarantee covers the use of ab77046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
Indirect ELISA Use at an assay dependent dilution.

靶标

  • 功能Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates transcription factor CREB1.
  • 组织特异性Mainly expressed in brain with small amounts in skeletal muscles, kidney, spleen and liver. Strongly expressed in forebrain neocortex, striatum and limbic system.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • 结构域The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.
  • 翻译后修饰May be prenylated on Cys-473.
  • 细胞定位Cytoplasm. Golgi apparatus membrane. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Calcium/calmodulin dependent protein kinase type 1G antibody
    • Calcium/calmodulin-dependent protein kinase I-gamma antibody
    • Calcium/calmodulin-dependent protein kinase Ig antibody
    • Calcium/calmodulin-dependent protein kinase type 1G antibody
    • CaM kinase I gamma antibody
    • CaM kinase IG antibody
    • CaM-KI gamma antibody
    • CaMK like CREB kinase III antibody
    • CaMK-like CREB kinase III antibody
    • Camk1g antibody
    • CaMKI gamma antibody
    • CaMKIG antibody
    • CLICK III antibody
    • CLICK3 antibody
    • dJ272L16.1 antibody
    • KCC1G_HUMAN antibody
    • VWS1 antibody
    see all

Anti-CaMKI gamma antibody 图像

  • All lanes : Anti-CaMKI gamma antibody (ab77046) at 1/500 dilution

    Lane 1 : CAMK1G-transfected 293T cell lysate (53.1 KDa)
    Lane 2 : non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP-conjugated goat anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
  • Anti-CaMKI gamma antibody (ab77046) at 1/500 dilution + immunogen (78.47 KDa) at 0.1 µg

    Secondary
    HRP-conjugated goat anti-mouse IgG at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 53 kDa
    Observed band size : 53 kDa

Anti-CaMKI gamma antibody (ab77046)参考文献

This product has been referenced in:
  • He Z  et al. TRPC5 channel is the mediator of neurotrophin-3 in regulating dendritic growth via CaMKIIa in rat hippocampal neurons. J Neurosci 32:9383-95 (2012). WB ; Rat . Read more (PubMed: 22764246) »

See 1 Publication for this product

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