Anti-CaMKI gamma抗体(ab38132)

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概述

  • 产品名称
    Anti-CaMKI gamma抗体
    参阅全部 CaMKI gamma 一抗
  • 描述
    兔多克隆抗体to CaMKI gamma
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Rat
    预测可用于: Mouse, Cow, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Mouse CaMKI gamma.

    参阅Abcam的专有抗源政策

    (Peptide available as ab38755.)

  • 阳性对照
    • This antibody gave a positive signal against Human recombinant CAMKI gamma kinase and PC12 cells.

性能

应用

Our Abpromise guarantee covers the use of ab38132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能
    Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates transcription factor CREB1.
  • 组织特异性
    Mainly expressed in brain with small amounts in skeletal muscles, kidney, spleen and liver. Strongly expressed in forebrain neocortex, striatum and limbic system.
  • 序列相似性
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • 结构域
    The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.
  • 翻译后修饰
    May be prenylated on Cys-473.
  • 细胞定位
    Cytoplasm. Golgi apparatus membrane. Cell membrane.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • Calcium/calmodulin dependent protein kinase type 1G antibody
    • Calcium/calmodulin-dependent protein kinase I-gamma antibody
    • Calcium/calmodulin-dependent protein kinase Ig antibody
    • Calcium/calmodulin-dependent protein kinase type 1G antibody
    • CaM kinase I gamma antibody
    • CaM kinase IG antibody
    • CaM-KI gamma antibody
    • CaMK like CREB kinase III antibody
    • CaMK-like CREB kinase III antibody
    • Camk1g antibody
    • CaMKI gamma antibody
    • CaMKIG antibody
    • CLICK III antibody
    • CLICK3 antibody
    • dJ272L16.1 antibody
    • KCC1G_HUMAN antibody
    • VWS1 antibody
    see all

图片

  • Western blot - Anti-CaMKI gamma antibody (ab38132)
    Western blot - Anti-CaMKI gamma antibody (ab38132)
    Anti-CaMKI gamma antibody (ab38132) at 1 µg/ml + Human recombinant CAMKI gamma kinase at 1 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 53 kDa
    Observed band size: 79 kDa (why is the actual band size different from the predicted?)



    Recombinant CAMKI gamma kinase is a fusion protein with a molecular weight of 79 kDa.
  • Immunocytochemistry/ Immunofluorescence - Anti-CaMKI gamma antibody (ab38132)
    Immunocytochemistry/ Immunofluorescence - Anti-CaMKI gamma antibody (ab38132)
    ICC/IF image of ab38132 stained rat PC12 cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab38132, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

文献

ab38132 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab38132？请让我们知道，以便我们可以引用本数据表中的参考文章。

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CaMKI gamma antibody

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
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Dr. Sophie Pezet

Verified customer

提交于 Jan 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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