概述
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产品名称Anti-CaMKI gamma抗体
参阅全部 CaMKI gamma 一抗 -
描述兔多克隆抗体to CaMKI gamma
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经测试应用适用于: WB, ICC/IFmore details
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种属反应性与反应: Rat
预测可用于: Mouse, Cow, Human
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Mouse CaMKI gamma.
(Peptide available as ab38755.)
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阳性对照
- This antibody gave a positive signal against Human recombinant CAMKI gamma kinase and PC12 cells.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab38132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa. | |
| ICC/IF | Use a concentration of 1 µg/ml. |
靶标
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功能Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates transcription factor CREB1.
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组织特异性Mainly expressed in brain with small amounts in skeletal muscles, kidney, spleen and liver. Strongly expressed in forebrain neocortex, striatum and limbic system.
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序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
结构域The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.
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翻译后修饰May be prenylated on Cys-473.
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细胞定位Cytoplasm. Golgi apparatus membrane. Cell membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 57172 Human
- Entrez Gene: 215303 Mouse
- Entrez Gene: 171358 Rat
- Omim: 614994 Human
- SwissProt: Q96NX5 Human
- SwissProt: Q91VB2 Mouse
- SwissProt: Q7TNJ7 Rat
- Unigene: 199068 Human
see all -
别名
- Calcium/calmodulin dependent protein kinase type 1G antibody
- Calcium/calmodulin-dependent protein kinase I-gamma antibody
- Calcium/calmodulin-dependent protein kinase Ig antibody
see all
Anti-CaMKI gamma antibody 图像
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Western blot - CaMKI gamma antibody (ab38132)Anti-CaMKI gamma antibody (ab38132) at 1 µg/ml + Human recombinant CAMKI gamma kinase at 1 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 53 kDa
Observed band size : 79 kDa (why is the actual band size different from the predicted?) -
Immunocytochemistry/ Immunofluorescence - CaMKI gamma antibody (ab38132)ICC/IF image of ab38132 stained rat PC12 cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab38132, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Anti-CaMKI gamma antibody (ab38132)参考文献
ab38132 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"