Anti-CaMKI gamma抗体(ab38132)


  • 产品名称Anti-CaMKI gamma抗体
    参阅全部 CaMKI gamma 一抗
  • 描述
    兔多克隆抗体to CaMKI gamma
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Rat
    预测可用于: Mouse, Cow, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Mouse CaMKI gamma.

    (Peptide available as ab38755.)

  • 阳性对照
    • This antibody gave a positive signal against Human recombinant CAMKI gamma kinase and PC12 cells.



Our Abpromise guarantee covers the use of ab38132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa.
ICC/IF Use a concentration of 1 µg/ml.


  • 功能Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates transcription factor CREB1.
  • 组织特异性Mainly expressed in brain with small amounts in skeletal muscles, kidney, spleen and liver. Strongly expressed in forebrain neocortex, striatum and limbic system.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • 结构域The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.
  • 翻译后修饰May be prenylated on Cys-473.
  • 细胞定位Cytoplasm. Golgi apparatus membrane. Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Calcium/calmodulin dependent protein kinase type 1G antibody
    • Calcium/calmodulin-dependent protein kinase I-gamma antibody
    • Calcium/calmodulin-dependent protein kinase Ig antibody
    • Calcium/calmodulin-dependent protein kinase type 1G antibody
    • CaM kinase I gamma antibody
    • CaM kinase IG antibody
    • CaM-KI gamma antibody
    • CaMK like CREB kinase III antibody
    • CaMK-like CREB kinase III antibody
    • Camk1g antibody
    • CaMKI gamma antibody
    • CaMKIG antibody
    • CLICK III antibody
    • CLICK3 antibody
    • dJ272L16.1 antibody
    • KCC1G_HUMAN antibody
    • VWS1 antibody
    see all

Anti-CaMKI gamma antibody 图像

  • Anti-CaMKI gamma antibody (ab38132) at 1 µg/ml + Human recombinant CAMKI gamma kinase at 1 µg

    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 79 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab38132 stained rat PC12 cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab38132, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

Anti-CaMKI gamma antibody (ab38132)参考文献

ab38132 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No

Dr. Sophie Pezet

Verified customer

提交于 Jan 15 2010