产品名称 Anti-CaMKI gamma抗体 参阅全部 CaMKI gamma 一抗
兔多克隆抗体to CaMKI gamma
WB, ICC/IF more details
Mouse, Cow, Human
Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Mouse CaMKI gamma.
(Peptide available as
This antibody gave a positive signal against Human recombinant CAMKI gamma kinase and PC12 cells.
存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液 Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa.
Use a concentration of 1 µg/ml.
功能 Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates transcription factor CREB1.
组织特异性 Mainly expressed in brain with small amounts in skeletal muscles, kidney, spleen and liver. Strongly expressed in forebrain neocortex, striatum and limbic system.
序列相似性 Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain.
结构域 The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.
翻译后修饰 May be prenylated on Cys-473.
细胞定位 Cytoplasm. Golgi apparatus membrane. Cell membrane.
Information by UniProt
Calcium/calmodulin dependent protein kinase type 1G antibody
Calcium/calmodulin-dependent protein kinase I-gamma antibody
Calcium/calmodulin-dependent protein kinase Ig antibody
Anti-CaMKI gamma antibody 图像
Western blot - CaMKI gamma antibody (ab38132)
Anti-CaMKI gamma antibody (ab38132) at 1 µg/ml + Human recombinant CAMKI gamma kinase at 1 µg Secondary IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution Performed under reducing conditions. Predicted band size : 53 kDa Observed band size : 79 kDa ( why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - CaMKI gamma antibody (ab38132)
ICC/IF image of ab38132 stained rat PC12 cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab38132, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Anti-CaMKI gamma antibody (ab38132)参考文献
has not yet been referenced specifically in any publications.
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