概述

  • 产品名称Anti-CaMKI抗体[EPR2217Y]
    参阅全部 CaMKI 一抗
  • 描述
    兔单克隆抗体[EPR2217Y] to CaMKI
  • 经测试应用适用于: WB, IHC-P, ICCmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A specific peptide corresponding to residues on the N-terminus of human CaMKI.

  • 阳性对照
    • SH-SY5Y lysate, human, mouse and rat brain lysate and human kidney tissue sections.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

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应用

Our Abpromise guarantee covers the use of ab68234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
IHC-P 1/100 - 1/250.
ICC 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca(2+) elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser-694', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser-498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen-stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation (By similarity). Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K(+) and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I.
    • 组织特异性Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex.
    • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
      Contains 1 protein kinase domain.
    • 结构域The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.
    • 翻译后修饰Phosphorylated by CaMKK1 and CaMKK2 on Thr-177.
      Polybiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL12), leading to proteasomal degradation.
    • 细胞定位Cytoplasm. Nucleus. Predominantly cytoplasmic.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Calcium / calmodulin dependent protein kinase 1 antibody
      • Calcium / calmodulin dependent protein kinase I antibody
      • Calcium / calmodulin dependent protein kinase type 1 antibody
      • Calcium/calmodulin dependent protein kinase 1 antibody
      • Calcium/calmodulin dependent protein kinase I antibody
      • Calcium/calmodulin dependent protein kinase type 1 antibody
      • Calcium/calmodulin-dependent protein kinase type 1 antibody
      • CaM K1 antibody
      • CaM KI antibody
      • CaM kinase 1 alpha antibody
      • CAM kinase 1 antibody
      • CaM kinase I alpha antibody
      • CaM kinase I antibody
      • CaM-KI antibody
      • CaMK 1 antibody
      • CAMK I antibody
      • CaMK1 alpha antibody
      • CAMK1 antibody
      • CAMK1 PEN antibody
      • CaMKI alpha antibody
      • CaMKI-alpha antibody
      • KCC1A_HUMAN antibody
      • MGC120317 antibody
      • MGC120318 antibody
      see all

    Anti-CaMKI antibody [EPR2217Y] 图像

    • All lanes : Anti-CaMKI antibody [EPR2217Y] (ab68234) at 1/5000 dilution

      Lane 1 : SH-SY5Y lysate
      Lane 2 : human brain tissue lysate
      Lane 3 : mouse brain tissue lysate
      Lane 4 : rat brain tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 41 kDa
      Observed band size : 41 kDa
    • ab68234 at 1/100-1/250 dilution staining CaMKI in human kidney tissue sections.

    Anti-CaMKI antibody [EPR2217Y] (ab68234)参考文献

    This product has been referenced in:
    • Yan X  et al. CaMKII-Mediated CREB Phosphorylation Is Involved in Ca2+-Induced BDNF mRNA Transcription and Neurite Outgrowth Promoted by Electrical Stimulation. PLoS One 11:e0162784 (2016). WB, ICC/IF ; Rat . Read more (PubMed: 27611779) »
    • Elzière L  et al. CaMKK-CaMK1a, a new post-traumatic signalling pathway induced in mouse somatosensory neurons. PLoS One 9:e97736 (2014). ICC/IF ; Mouse . Read more (PubMed: 24840036) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (liver)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH6.0
    Permeabilization No
    Specification liver
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative Formaldehyde
    Username

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    Verified customer

    提交于 Aug 19 2016

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing
    Sample Mouse Tissue lysate - whole (liver)
    Specification liver
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    提交于 Dec 26 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"