重组Anti-CaMKI抗体[EPR2217Y] (ab68234)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2217Y] to CaMKI
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-CaMKI抗体[EPR2217Y]
参阅全部 CaMKI 一抗 -
描述
兔单克隆抗体[EPR2217Y] to CaMKI -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human CaMKI (N terminal). The exact sequence is proprietary.
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阳性对照
- SH-SY5Y lysate, human, mouse and rat brain lysate and human kidney tissue sections.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2217Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab68234于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (3) |
1/5000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
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IHC-P | (1) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/5000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca(2+) elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser-694', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser-498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen-stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation (By similarity). Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K(+) and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I. -
组织特异性
Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
结构域
The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate. -
翻译后修饰
Phosphorylated by CaMKK1 and CaMKK2 on Thr-177.
Polybiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL12), leading to proteasomal degradation. -
细胞定位
Cytoplasm. Nucleus. Predominantly cytoplasmic. - Information by UniProt
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数据库链接
- Entrez Gene: 8536 Human
- Entrez Gene: 52163 Mouse
- Entrez Gene: 171503 Rat
- Omim: 604998 Human
- SwissProt: Q14012 Human
- SwissProt: Q91YS8 Mouse
- SwissProt: Q63450 Rat
- Unigene: 434875 Human
see all -
别名
- Calcium / calmodulin dependent protein kinase 1 antibody
- Calcium / calmodulin dependent protein kinase I antibody
- Calcium / calmodulin dependent protein kinase type 1 antibody
see all
图片
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All lanes : Anti-CaMKI antibody [EPR2217Y] (ab68234)
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : Human brain tissue lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast)
Lane 5 : Rat brain tissue lysate
Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDaExposure time: Lane 1 & 3, 4, 5: 5 seconds. Lane 2 & 6:20 seconds
Blocking and diluting buffer: 5% NFDM /TBST
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All lanes : Anti-CaMKI antibody [EPR2217Y] (ab68234) at 1/5000 dilution
Lane 1 : SH-SY5Y lysate
Lane 2 : human brain tissue lysate
Lane 3 : mouse brain tissue lysate
Lane 4 : rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa -
Immunohistochemical analysis of Paraffin-embedded Human adrenal gland tissue labelling CaMKI with ab68234 at 1/500 dilution, followed by ready to use secondary antibody Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on the zona glomerulosa of human adrenal glands. The section was incubated with ab68234 at +4°C overnight. Counter stained with Hematoxylin.
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ab68234 at 1/100-1/250 dilution staining CaMKI in human kidney tissue sections.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab68234 被引用在 13 文献中.
- Yang J et al. Pitavastatin activates mitophagy to protect EPC proliferation through a calcium-dependent CAMK1-PINK1 pathway in atherosclerotic mice. Commun Biol 5:124 (2022). PubMed: 35145192
- Hu C et al. The low levels of nerve growth factor and its upstream regulatory kinases in prion infection is reversed by resveratrol. Neurosci Res 162:52-62 (2021). PubMed: 31891740
- Lei Y et al. Expression of CAMK1 and its association with clinicopathologic characteristics in pancreatic cancer. J Cell Mol Med 25:1198-1206 (2021). PubMed: 33342045
- Bai L et al. Mechanisms Underlying the Antidepressant Effect of Acupuncture via the CaMK Signaling Pathway. Front Behav Neurosci 14:563698 (2020). PubMed: 33343309
- Li M et al. Transient Receptor Potential V Channels Are Essential for Glucose Sensing by Aldolase and AMPK. Cell Metab 30:508-524.e12 (2019). PubMed: 31204282