The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/20000 - 1/50000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
1/100 - 1/250.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
应用说明Is unsuitable for IHC-P.
功能Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca(2+) elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser-694', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser-498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen-stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation (By similarity). Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K(+) and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I.
组织特异性Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex.
序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain.
结构域The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.
翻译后修饰Phosphorylated by CaMKK1 and CaMKK2 on Thr-177. Polybiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL12), leading to proteasomal degradation.
Calcium / calmodulin dependent protein kinase 1 antibody
Calcium / calmodulin dependent protein kinase I antibody
Calcium / calmodulin dependent protein kinase type 1 antibody
Calcium/calmodulin dependent protein kinase 1 antibody
Calcium/calmodulin dependent protein kinase I antibody
Calcium/calmodulin dependent protein kinase type 1 antibody
Calcium/calmodulin-dependent protein kinase type 1 antibody
CaM K1 antibody
CaM KI antibody
CaM kinase 1 alpha antibody
CAM kinase 1 antibody
CaM kinase I alpha antibody
CaM kinase I antibody
CaMK 1 antibody
CAMK I antibody
CaMK1 alpha antibody
CAMK1 PEN antibody
CaMKI alpha antibody
Anti-CaMKI antibody [EP2218Y] 图像
Western blot - CaMKI antibody [EP2218Y] (ab62374)
Anti-CaMKI antibody [EP2218Y] (ab62374) at 1/20000 dilution + SH-SY5Y cell lysate at 10 µg
Secondary goat anti-rabbit, HRP labeled, at 1/2000 dilution
Predicted band size : 41 kDa Observed band size : 41 kDa
Anti-CaMKI antibody [EP2218Y] (ab62374)参考文献
This product has been referenced in:
Pfisterer SG et al. Ca2+/calmodulin-dependent kinase (CaMK) signaling via CaMKI and AMP-activated protein kinase contributes to the regulation of WIPI-1 at the onset of autophagy. Mol Pharmacol80:1066-75 (2011).
Read more (PubMed: 21896713) »