Anti-CaMKI抗体- Carboxyterminal end (ab38002)

概述

  • 产品名称Anti-CaMKI抗体- Carboxyterminal end
    参阅全部 CaMKI 一抗
  • 描述
    兔多克隆抗体to CaMKI - Carboxyterminal end
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human CaMKI aa 302-333 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • 阳性对照
    • NCIH460 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab38002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IHC-P 1/50 - 1/100.

靶标

  • 功能Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Regulates axonal extension and growth cone motility in hippocampal and cerebellar nerve cells. Upon NMDA receptor-mediated Ca(2+) elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. Downstream of NMDA receptors, promotes the formation of spines and synapses in hippocampal neurons by phosphorylating ARHGEF7/BETAPIX on 'Ser-694', which results in the enhancement of ARHGEF7 activity and activation of RAC1. Promotes neuronal differentiation and neurite outgrowth by activation and phosphorylation of MARK2 on 'Ser-91', 'Ser-92', 'Ser-93' and 'Ser-294'. Promotes nuclear export of HDAC5 and binding to 14-3-3 by phosphorylation of 'Ser-259' and 'Ser-498' in the regulation of muscle cell differentiation. Regulates NUMB-mediated endocytosis by phosphorylation of NUMB on 'Ser-276' and 'Ser-295'. Involved in the regulation of basal and estrogen-stimulated migration of medulloblastoma cells through ARHGEF7/BETAPIX phosphorylation (By similarity). Is required for proper activation of cyclin-D1/CDK4 complex during G1 progression in diploid fibroblasts. Plays a role in K(+) and ANG2-mediated regulation of the aldosterone synthase (CYP11B2) to produce aldosterone in the adrenal cortex. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CFTR, MYL9 and SYN1/synapsin I.
  • 组织特异性Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • 结构域The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.
  • 翻译后修饰Phosphorylated by CaMKK1 and CaMKK2 on Thr-177.
    Polybiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL12), leading to proteasomal degradation.
  • 细胞定位Cytoplasm. Nucleus. Predominantly cytoplasmic.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Calcium / calmodulin dependent protein kinase 1 antibody
    • Calcium / calmodulin dependent protein kinase I antibody
    • Calcium / calmodulin dependent protein kinase type 1 antibody
    • Calcium/calmodulin dependent protein kinase 1 antibody
    • Calcium/calmodulin dependent protein kinase I antibody
    • Calcium/calmodulin dependent protein kinase type 1 antibody
    • Calcium/calmodulin-dependent protein kinase type 1 antibody
    • CaM K1 antibody
    • CaM KI antibody
    • CaM kinase 1 alpha antibody
    • CAM kinase 1 antibody
    • CaM kinase I alpha antibody
    • CaM kinase I antibody
    • CaM-KI antibody
    • CaMK 1 antibody
    • CAMK I antibody
    • CaMK1 alpha antibody
    • CAMK1 antibody
    • CAMK1 PEN antibody
    • CaMKI alpha antibody
    • CaMKI-alpha antibody
    • KCC1A_HUMAN antibody
    • MGC120317 antibody
    • MGC120318 antibody
    see all

Anti-CaMKI antibody - Carboxyterminal end 图像

  • Anti-CaMKI antibody - Carboxyterminal end (ab38002) at 1/100 dilution + NCIH460 cell lysate.
    Developed using the ECL technique

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
  • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with 1/50 ab38002.

Anti-CaMKI antibody - Carboxyterminal end (ab38002)参考文献

ab38002 has not yet been referenced specifically in any publications.

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