Synthetic peptide corresponding to Human CAMK2A aa 400 to the C-terminus conjugated to keyhole limpet haemocyanin. (Peptide available as ab115931)
This antibody gave a positive signal in the following tissue lysates: Mouse brain; Rat brain; Mouse cortex; Rat cortex.
IHC-P: FFPE rat brain tissue sections.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
IHC image of CAMK2A staining in rat brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab103840, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-CAM2KA antibody (ab103840)
All lanes : Anti-CAMK2A antibody (ab103840) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate Lane 2 : Brain (Rat) Tissue Lysate Lane 3 : Mouse cortex Mouse Tissue Lysate Lane 4 : Rat Cortex Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique