重组
RabMAb

Anti-Calsequestrin 2抗体[EPR4228] (ab108381)

概述

  • 产品名称
    Anti-Calsequestrin 2抗体[EPR4228]
    参阅全部 Calsequestrin 2 一抗
  • 描述
    兔单克隆抗体[EPR4228] to Calsequestrin 2
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    corresponding to Human Calsequestrin 2 aa 200-300.

  • 阳性对照
    • Human heart and Human skeletal muscle lysates, Human cardiac muscles and Human skeletal muscles tissues
  • 常规说明

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab108381 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 46 kDa).
IHC-P 1/100 - 1/250.

Perform antigen retrieval before commencing with IHC staining protocol.
See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

靶标

  • 功能
    Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. The release of calcium bound to calsequestrin through a calcium release channel triggers muscle contraction. Binds 40 to 50 moles of calcium.
  • 疾病相关
    Defects in CASQ2 are the cause of catecholaminergic polymorphic ventricular tachycardia type 2 (CPVT2) [MIM:611938]; also known as stress-induced polymorphic ventricular tachycardia (VTSIP). CPVT2 is an autosomal recessive form of arrhythmogenic disorder characterized by stress-induced, bidirectional ventricular tachycardia that may degenerate into cardiac arrest and cause sudden death.
  • 序列相似性
    Belongs to the calsequestrin family.
  • 细胞定位
    Sarcoplasmic reticulum lumen. This isoform of calsequestrin occurs in the sarcoplasmic reticulum's terminal cisternae luminal spaces of cardiac and slow skeletal muscle cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AA033488 antibody
    • AW146219 antibody
    • Calsequestrin 2 (cardiac muscle) antibody
    • Calsequestrin 2 fast twitch cardiac muscle antibody
    • Calsequestrin antibody
    • Calsequestrin cardiac muscle isoform antibody
    • Calsequestrin fast twitch cardiac muscle antibody
    • Calsequestrin-2 antibody
    • Calsequestrin2 antibody
    • cardCSQ antibody
    • Cardiac calsequestrin 2 antibody
    • cardiac muscle isoform antibody
    • CASQ 2 antibody
    • CASQ2 antibody
    • CASQ2_HUMAN antibody
    • cCSQ antibody
    • ESTM52 antibody
    • FLJ26321 antibody
    • FLJ93514 antibody
    • PDIB2 antibody
    see all

图片

  • Immunohistochemical analysis of paraffin-embedded Human skeletal muscles using unpurified ab108381 at a dilution of 1/100.

  • All lanes : Anti-Calsequestrin 2 antibody [EPR4228] (ab108381) at 1/10000 dilution (purified)

    Lane 1 : Human fetal heart lysates
    Lane 2 : Human skeletal muscle lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size : 46 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

    Blocking and diluting buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue sections labeling Calsequestrin 2 with Purified ab108381 at 1:250 dilution (0.5 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • All lanes : Anti-Calsequestrin 2 antibody [EPR4228] (ab108381) at 1/1000 dilution (unpurified)

    Lane 1 : Human heart lysate
    Lane 2 : Human skeletal muscle lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 46 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin-embedded Human cardiac muscles using unpurified ab108381 at a dilution of 1/100.

  • Unpurified ab108381 showing negative staining in Normal brain tissue.

  • Unpurified ab108381 showing negative staining in Normal breast tissue.

  • Unpurified ab108381 showing negative staining in Normal kidney tissue.

  • Unpurified ab108381 showing negative staining in Normal colon tissue.

  • Unpurified ab108381 showing negative staining in Normal tonsil tissue.

文献

ab108381 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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