概述

  • 产品名称Anti-Calretinin抗体[SP13]
    参阅全部 Calretinin 一抗
  • 描述
    兔单克隆抗体[SP13] to Calretinin
  • 特异性Ab16694 recognises Calretinin.
  • 经测试应用适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    Recombinant full length calretinin protein (Mouse).

  • 阳性对照
    • Human mesothelioma for Immunohistochemistry.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide.
    Constituents: Tissue culture supernatant.
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号SP13
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab16694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/200.

靶标

Anti-Calretinin antibody [SP13] 图像

  • Immunocytochemical analysis of 5% PFA & 0.2% Picric Acid fixed rat cordical cell sections, labelling Calretinin with ab16694 at a dilution of 1/10 incubated fro 12 hours at 4°C in a 10mM PBS & 0.35% Triton X diluent. Secondary was a donkey anti-rabbit polyclonal Alexa Fluor® 488 conjugate at 1/200.

    See Abreview

  • Ab16694 at a dilution of 1/100, staining Calretinin in formalin fixed paraffin embedded human mesothelioma tissue section by Immunohistochemistry.

Anti-Calretinin antibody [SP13] (ab16694)参考文献

This product has been referenced in:
  • Kuwabara T  et al. Wnt-mediated activation of NeuroD1 and retro-elements during adult neurogenesis. Nat Neurosci 12:1097-105 (2009). ICC/IF ; Rat . Read more (PubMed: 19701198) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Rat Cortical Cell Culture)
Permeabilization No
Specification Rat Cortical Cell Culture
Fixative 5% PF, 02% Picric Acid
Username

Ms. Babben Tinner

Verified customer

提交于 Oct 07 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"