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Our Abpromise guarantee covers the use of ab16694 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Immunocytochemical analysis of 5% PFA & 0.2% Picric Acid fixed rat cordical cell sections, labelling Calretinin with ab16694 at a dilution of 1/10 incubated fro 12 hours at 4°C in a 10mM PBS & 0.35% Triton X diluent. Secondary was a donkey anti-rabbit polyclonal Alexa Fluor® 488 conjugate at 1/200.
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