重组Anti-Calretinin抗体[SP13] (ab16694)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP13] to Calretinin
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Calretinin抗体[SP13]
参阅全部 Calretinin 一抗 -
描述
兔单克隆抗体[SP13] to Calretinin -
宿主
Rabbit -
经测试应用
适用于: IHC-P, IHC-Frmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant full length protein within Mouse Calretinin. The exact sequence is proprietary.
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阳性对照
- IHC-P: Human mesothelioma. IHC-Fr: Mouse and rat cerebrum tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, 98.9% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
SP13 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab16694于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (1) |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
IHC-Fr |
1/30.
|
说明 |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
IHC-Fr
1/30. |
靶标
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功能
Calretinin is a calcium-binding protein which is abundant in auditory neurons. -
组织特异性
Brain. -
序列相似性
Belongs to the calbindin family.
Contains 6 EF-hand domains. - Information by UniProt
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数据库链接
- Entrez Gene: 794 Human
- Entrez Gene: 12308 Mouse
- Entrez Gene: 117059 Rat
- Omim: 114051 Human
- SwissProt: P22676 Human
- SwissProt: Q08331 Mouse
- SwissProt: P47728 Rat
- Unigene: 106857 Human
see all -
别名
- 29 kDa calbindin antibody
- CAB 29 antibody
- CAB29 antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded Human mesothelioma labeling Calretinin with ab16694 at 1/100 (27 µg/ml). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16694 for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. DAB was used as the chromogen. Counterstained with Hematoxylin and mounted with DPX.
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This image was generated using a previous batch manufactured using hybridoma production method.
Immunohistochemistry (Frozen) analysis of mouse cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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This image was generated using a previous batch manufactured using hybridoma production method.
Immunohistochemistry (Frozen) analysis of rat cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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This image was generated using a previous batch manufactured using hybridoma production method.
Immunohistochemistry (Frozen) analysis of rat cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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This image was generated using a previous batch manufactured using hybridoma production method.
Immunohistochemistry (Frozen) analysis of mouse cerebrum tissue section labeling Calretinin with purified ab16694 at 1/30 (9.6 µg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab16694 被引用在 10 文献中.
- Shi CJ et al. TGFβR-1/ALK5 inhibitor RepSox induces enteric glia-to-neuron transition and influences gastrointestinal mobility in adult mice. Acta Pharmacol Sin 44:92-104 (2023). PubMed: 35794374
- Pruett N et al. Normal mesothelial cell lines newly derived from human pleural biopsy explants. Am J Physiol Lung Cell Mol Physiol 319:L652-L660 (2020). PubMed: 32726133
- Murayama AY et al. The polymicrogyria-associated GPR56 promoter preferentially drives gene expression in developing GABAergic neurons in common marmosets. Sci Rep 10:21516 (2020). PubMed: 33299078
- Sakaguchi H et al. Self-Organized Synchronous Calcium Transients in a Cultured Human Neural Network Derived from Cerebral Organoids. Stem Cell Reports 13:458-473 (2019). PubMed: 31257131
- Ohara Y et al. Connective tissue growth factor-specific monoclonal antibody inhibits growth of malignant mesothelioma in an orthotopic mouse model. Oncotarget 9:18494-18509 (2018). PubMed: 29719620
- Yin M et al. Gremlin-1 is a key regulator of the invasive cell phenotype in mesothelioma. Oncotarget 8:98280-98297 (2017). PubMed: 29228689
- Roulois D et al. Characterization of preneoplastic and neoplastic rat mesothelial cell lines: the involvement of TETs, DNMTs, and 5-hydroxymethylcytosine. Oncotarget 7:34664-87 (2016). PubMed: 27129173
- Aierken D et al. Rat model demonstrates a high risk of tremolite but a low risk of anthophyllite for mesothelial carcinogenesis. Nagoya J Med Sci 76:149-60 (2014). PubMed: 25130001
- Lin B et al. Forebrain ischemia triggers GABAergic system degeneration in substantia nigra at chronic stages in rats. Cardiovasc Psychiatry Neurol 2010:506952 (2010). IHC ; Rat . PubMed: 20981346
- Kuwabara T et al. Wnt-mediated activation of NeuroD1 and retro-elements during adult neurogenesis. Nat Neurosci 12:1097-105 (2009). ICC/IF ; Rat . PubMed: 19701198