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Our Abpromise guarantee covers the use of ab702 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For an ABC system, dilute 1/50-1/100 and incubate for 30-60 minutes at RT.
|IHC-Fr||1/100. PubMed: 20504962
Immunohistochemical studies have recently shown calretinin to be useful in distinguishing mesotheliomas from lung adenocarcinomas. However, it is recommended that a panel of antibodies be used in tandem with calretinin. Other antibodies recommended are CK 5/6, E-cadherin, ESA or BerEP4, CEA, EMA, B72.3, Vimentin and CD15. Calretinin does not mark all mesotheliomas (50-70%).
ab702 staining Calretinin in Mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 3% serum for 30 minutes at 27°C. Samples were incubated with primary antibody (1/250) for 20 hours at 4°C. A Cy3®-conjugated donkey anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
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