Anti-Calreticulin抗体[FMC 75] (Phycoerythrin) (ab83220)

概述

  • 产品名称
    Anti-Calreticulin抗体[FMC 75] (Phycoerythrin)
    参阅全部 Calreticulin 一抗
  • 描述
    小鼠单克隆抗体[FMC 75] to Calreticulin (Phycoerythrin)
  • 偶联物
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • 经测试应用
    适用于: Flow Cytmore details
  • 种属反应性
    与反应: Cow, Human, Monkey
  • 免疫原

    Recombinant Calreticulin protein

  • 阳性对照
    • Jurkat cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    FMC 75
  • 同种型
    IgG1
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab83220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/100. ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
  • 序列相似性
    Belongs to the calreticulin family.
  • 结构域
    Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
    The interaction with glycans occurs through a binding site in the globular lectin domain.
    The zinc binding sites are localized to the N-domain.
    Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
  • 细胞定位
    Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Autoantigen RO antibody
    • CALR antibody
    • CALR protein antibody
    • CALR_HUMAN antibody
    • Calregulin antibody
    • Calreticulin antibody
    • cC1qR antibody
    • CRP55 antibody
    • CRT antibody
    • CRTC antibody
    • Endoplasmic reticulum resident protein 60 antibody
    • Epididymis secretory sperm binding protein Li 99n antibody
    • ERp60 antibody
    • FLJ26680 antibody
    • grp60 antibody
    • HACBP antibody
    • HEL S 99n antibody
    • RO antibody
    • Sicca syndrome antigen A (autoantigen Ro; calreticulin) antibody
    • Sicca syndrome antigen A antibody
    • SSA antibody
    see all

图片

  • Intracellular staining of 106 Jurkat cells using ab83220 at a concentration of 10µg/ml.

文献

This product has been referenced in:
  • Draganov D  et al. Modulation of P2X4/P2X7/Pannexin-1 sensitivity to extracellular ATP via Ivermectin induces a non-apoptotic and inflammatory form of cancer cell death. Sci Rep 5:16222 (2015). Read more (PubMed: 26552848) »
  • Angelova AL  et al. Complementary induction of immunogenic cell death by oncolytic parvovirus H-1PV and gemcitabine in pancreatic cancer. J Virol 88:5263-76 (2014). Flow Cyt ; Human . Read more (PubMed: 24574398) »

See all 3 Publications for this product

客户评价及客户问答

Application
Flow Cytometry
Sample
Mouse Cell (B16 melanoma)
Permeabilization
Yes - BD Perm/Fix kit was used for intracellular staining
Gating Strategy
Greater than 90% of cells were calreticulin+ compared to PE Isotype control
Specification
B16 melanoma
Preparation
Cell harvesting/tissue preparation method: Cells were trypinised to obtain a single cell suspension.
Sample buffer: Perm/Fix Buffer
Fixation
Paraformaldehyde
Username

Felicia Cao

Verified customer

提交于 Jul 27 2015

According to http://www.uniprot.org/uniprot/P27797 Calreticulin is localized to the ER, cytoplasm, secreted, and on the cell surface, so rather than being a multipass membrane protein it is translocated to different regions of the cell. Thus, uniprot d...

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The epitope that this calreticulin antibody recognizes has not been mapped but it is expected that the epitope is on an extracellular region. The unconjugated version of this clone, ab22863, was used for staining live cells for flow cytometry in PubMed...

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The immunogen for ab83220 is the full length recombinant protein.

Vielen Dank für Ihren Anruf.

Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten. Wie am Telefon besprochen habe ich eine kostenlose Ersatzlieferung mit einer anderen Lot# in Auftrag gegeben. Sie hat die Referenznummer 1...

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Thank you for your enquiry regarding ab22683.

We have removed Hamster from the "does not react" species list, this seems to be an error.

We apologize for any inconvenience caused and thank you for bringing this matter to o...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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