• 产品名称Anti-Calreticulin抗体
    参阅全部 Calreticulin 一抗
  • 描述
    兔多克隆抗体to Calreticulin
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    synthesized peptide derived from an internal region of human Calreticulin

  • 阳性对照
    • Extracts from COS7 cells. Heart tissue.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab70013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
ELISA 1/40000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.


  • 功能Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
  • 序列相似性Belongs to the calreticulin family.
  • 结构域Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
    The interaction with glycans occurs through a binding site in the globular lectin domain.
    The zinc binding sites are localized to the N-domain.
    Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
  • 细胞定位Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Autoantigen RO antibody
    • CALR antibody
    • CALR protein antibody
    • CALR_HUMAN antibody
    • Calregulin antibody
    • Calreticulin antibody
    • cC1qR antibody
    • CRP55 antibody
    • CRT antibody
    • CRTC antibody
    • Endoplasmic reticulum resident protein 60 antibody
    • Epididymis secretory sperm binding protein Li 99n antibody
    • ERp60 antibody
    • FLJ26680 antibody
    • grp60 antibody
    • HACBP antibody
    • HEL S 99n antibody
    • RO antibody
    • Sicca syndrome antigen A antibody
    • SSA antibody
    see all

Anti-Calreticulin antibody 图像

  • All lanes : Anti-Calreticulin antibody (ab70013) at 1/500 dilution

    Lane 1 : extracts from COS7 cells
    Lane 2 : extracts from COS7 cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
    Additional bands at : 110 kDa,19 kDa,35 kDa. We are unsure as to the identity of these extra bands.
  • immunohistochemistry analysis of paraffin-embedded human heart tissue using 1/50 ab70013. Right: with pretreatment of the antibody with the immunizing peptide

Anti-Calreticulin antibody (ab70013)参考文献

ab70013 has not yet been referenced specifically in any publications.

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Thank you for contacting us. I have looked through our catalog for the products against the targets that you have chosen that either have been specifically tested in ELISA or are guaranteed to work in ELISA by us. My findings are as follows:

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Following experiments related to ELISA have been carried out: 1) immunogen peptide ELISA--coat immunogen peptide to 96-well plate and detect with ab70013 with/without peptide preincubation. 2) cell-based ELISA--grow cells in 96-well plate, fix the ce...

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