Anti-Calreticulin抗体(ab4109)

概述

  • 产品名称Anti-Calreticulin抗体
    参阅全部 Calreticulin 一抗
  • 描述
    山羊多克隆抗体to Calreticulin
  • 特异性Recognises calreticulin.
  • 经测试应用适用于: WB, IHC-Frmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Human
  • 免疫原

    Purified rabbit skeletal muscle calreticulin

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液glycine buffered saline pH7.4, 0.1% azide
  • 纯度Whole antiserum
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab4109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/300 - 1/500.
IHC-Fr 1/50 - 1/70.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
    • 序列相似性Belongs to the calreticulin family.
    • 结构域Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
      The interaction with glycans occurs through a binding site in the globular lectin domain.
      The zinc binding sites are localized to the N-domain.
      Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
    • 细胞定位Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Autoantigen RO antibody
      • CALR antibody
      • CALR protein antibody
      • CALR_HUMAN antibody
      • Calregulin antibody
      • Calreticulin antibody
      • cC1qR antibody
      • CRP55 antibody
      • CRT antibody
      • CRTC antibody
      • Endoplasmic reticulum resident protein 60 antibody
      • Epididymis secretory sperm binding protein Li 99n antibody
      • ERp60 antibody
      • FLJ26680 antibody
      • grp60 antibody
      • HACBP antibody
      • HEL S 99n antibody
      • RO antibody
      • Sicca syndrome antigen A antibody
      • SSA antibody
      see all

    Anti-Calreticulin antibody (ab4109)参考文献

    ab4109 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Tissue lysate - whole (Heart)
    Gel Running Conditions Reduced Denaturing (12)
    Loading amount 20 µg
    Specification Heart
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Jul 01 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Heart)
    Gel Running Conditions Reduced Denaturing (12)
    Loading amount 20 µg
    Specification Heart
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Jun 01 2016

    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing (4-12)
    Sample Rat Tissue lysate - whole (liver)
    Specification liver
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Jul 23 2014

    Thank you for contacting us.

    The size of antibody as IgG is 150 kDa (25 kDa of each light chain and ˜53 kDa of each heavy chain).

    The size of protein as mentioned before is 417 amino acid 48kDa.

    I hope this information is hel...

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    The predicted size of unprocessed protein is 48kDa (http://www.uniprot.org/uniprot/P27797). The observed size will be higher than predicted because of post translational modifications at amino acid 48,159, 209 and ...

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    Thank you for contacting us.

    The predicted size of unprocessed protein is 48kDa (http://www.uniprot.org/uniprot/P27797). The observed size will be higher than predicted because of post translational modification at amino acid 48,159, 209 and ...

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    Thank you for contacting us.

    The antibody ab2907 was tried in Arabidopsis by a customer, and the results were submitted as an Abreview (2 out of 5 stars): http://www.abcam.com/index.html?datasheet=2907&tab=abreviews&intabreviewid=249...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (Brain)
    Specification Brain
    Fixative Acetone
    Permeabilization No
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    提交于 Jun 03 2011

    This antibody has not been tested for use in ELISA assays. As it is supplied as a serum, it would not be suitable for use as a coating antibody. There is no immunological reason why it could not be used as a detection antibody, but we cannot guarantee ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"