概述

  • 产品名称Anti-Calreticulin抗体
    参阅全部 Calreticulin 一抗
  • 描述
    兔多克隆抗体to Calreticulin
  • 特异性This antibody is specific for Calreticulin. It does not recognise other ER proteins.
  • 经测试应用适用于: WB, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human, Pig, Xenopus laevis
  • 免疫原

    Synthetic peptide:

    QAKDEL

    , corresponding to C terminal amino acids 412-417 of Human Calreticulin.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified using BSA-QAKKDEL attached to CNBr beads.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab15607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa.
ICC 1/50.

靶标

  • 功能Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
  • 序列相似性Belongs to the calreticulin family.
  • 结构域Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
    The interaction with glycans occurs through a binding site in the globular lectin domain.
    The zinc binding sites are localized to the N-domain.
    Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
  • 细胞定位Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Autoantigen RO antibody
    • CALR antibody
    • CALR protein antibody
    • CALR_HUMAN antibody
    • Calregulin antibody
    • Calreticulin antibody
    • cC1qR antibody
    • CRP55 antibody
    • CRT antibody
    • CRTC antibody
    • Endoplasmic reticulum resident protein 60 antibody
    • Epididymis secretory sperm binding protein Li 99n antibody
    • ERp60 antibody
    • FLJ26680 antibody
    • grp60 antibody
    • HACBP antibody
    • HEL S 99n antibody
    • RO antibody
    • Sicca syndrome antigen A antibody
    • SSA antibody
    see all

Anti-Calreticulin antibody 图像

  • Western blot using ab15607 at 1/1000 on mouse embyronic fibroblast lysate.

Anti-Calreticulin antibody (ab15607)参考文献

This product has been referenced in:
  • Saavedra MD  et al. Calreticulin from suboolemmal vesicles affects membrane regulation of polyspermy. Reproduction 147:369-78 (2014). WB ; Pig . Read more (PubMed: 24398873) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (purified fusion-protein with thioredoxin)
Loading amount 5 µg
Specification purified fusion-protein with thioredoxin
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Feb 09 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"