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Our Abpromise guarantee covers the use of ab700 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/100 - 1/400. ABC system. 30mins at RT.
Calponin has been found to be useful as a marker for myoepithelial and basal lamina in differentiating microinvasive from in situ ductal carcinomas of the breast. Calponin has also been useful in malignant myoepithelium and pleomorphoric adenoma of salivary gland as well as a useful marker for fine needle aspirates of papillary breast lesions.
|Flow Cyt||Use at an assay dependent concentration. PubMed: 20618694|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 20138661|
|IHC-P||1/100 - 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC system. 30mins at RT.|
ab700 staining Calponin in rat Smooth muscle cells from mesenteric artery by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 4% paraformaldehyde in physiological saline solution (PSS) for 4 min at 4°C and permeabilized with 0.3% Triton x100 before blocking with 2% BSA was done for 30 minutes at 20°C. Samples were incubated with primary antibody (1/300: in PSS with 2%BSA and 0.3% Triton X-100) for 14 hours at 4°C. An Alexa Fluor®488-conjugated chicken polyclonal to mouse IgG was used as secondary antibody at 1/400 dilution. Image shows combined fluorescent and transmitted light images. Hoechst 33342 was used to stain the cell nuclei (blue).