概述

  • 产品名称Anti-Calponin抗体
    参阅全部 Calponin 一抗
  • 描述
    兔多克隆抗体to Calponin
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human Calponin.

    (Peptide available as ab90216.)

  • 阳性对照
    • This antibody gave a positive signal in the following Human tissue lysates: Small Intestine; Heart; Bladder; Colon.

性能

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应用

Our Abpromise guarantee covers the use of ab78491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 33 kDa).

靶标

  • 功能Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
  • 组织特异性Smooth muscle, and tissues containing significant amounts of smooth muscle.
  • 序列相似性Belongs to the calponin family.
    Contains 3 calponin-like repeats.
    Contains 1 CH (calponin-homology) domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Basic calponin antibody
    • Calponin 1 antibody
    • Calponin 1 basic smooth muscle antibody
    • Calponin H1 antibody
    • Calponin H1 smooth muscle antibody
    • Calponin-1 antibody
    • Calponin1 antibody
    • Calponins basic antibody
    • CNN 1 antibody
    • Cnn1 antibody
    • CNN1_HUMAN antibody
    • Epididymis secretory protein Li 14 antibody
    • HEL S 14 antibody
    • Sm Calp antibody
    • SMCC antibody
    • smooth muscle antibody
    see all

Anti-Calponin antibody 图像

  • All lanes : Anti-Calponin antibody (ab78491) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 3 : Human bladder tissue lysate - soluble fraction (female, 45 years) (ab44654)
    Lane 4 : Human colon tissue lysate - total protein (ab30051)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 33 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes
  • IHC image of ab78491 staining in Human Normal Breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78491, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Calponin antibody (ab78491)参考文献

ab78491 has not yet been referenced specifically in any publications.

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