Anti-Calpastatin抗体(ab88065)

概述

  • 产品名称Anti-Calpastatin抗体
    参阅全部 Calpastatin 一抗
  • 描述
    小鼠单克隆抗体to Calpastatin
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Recombinant Fragment
    预测可用于: Human
  • 免疫原

    Recombinant fragment: QPDPDENKPM EDKVKEKAKA EHRDKLGERD DTIPPEYRHL LDDNGQDKPV, corresponding to amino acids 582-670 (NP_001741) of Human Calpastatin with proprietary tag.

  • 阳性对照
    • NIH 3T3 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab88065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 76 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
  • 序列相似性Belongs to the protease inhibitor I27 (calpastatin) family.
  • 结构域Has four inhibitory domains.
  • 翻译后修饰The N-terminus is blocked.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BS 17 antibody
    • Calpain inhibitor antibody
    • Calpastatin antibody
    • Cast antibody
    • Heart type calpastatin antibody
    • ICAL_HUMAN antibody
    • MGC9402 antibody
    • Sperm BS 17 component antibody
    • Sperm BS-17 component antibody
    see all

Anti-Calpastatin antibody 图像

  • Anti-Calpastatin antibody (ab88065) at 5 µg/ml + recombinant protein used as immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 76 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Anti-Calpastatin antibody (ab88065) at 5 µg/ml + NIH 3T3 cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 76 kDa
    Observed band size : 76 kDa

Anti-Calpastatin antibody (ab88065)参考文献

ab88065 has not yet been referenced specifically in any publications.

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