Anti-Calpastatin抗体(ab28252)

概述

  • 产品名称Anti-Calpastatin抗体
    参阅全部 Calpastatin 一抗
  • 描述
    兔多克隆抗体to Calpastatin
  • 特异性ab28252 recognises Calpastatin aminoterminal end (Domain L) testis isoform
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide to the amino terminal end of the testis isoform of human calpstatin.

性能

应用

Our Abpromise guarantee covers the use of ab28252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ICC/IF
  • 应用说明ICC/IF: Use at a concentration of 1 µg/ml.
    WB: 1/1000 when using colorimetric detection system (BCIP/NBT substrates); 1/5000 when using chemoluminescent substrates. Predicted molecular weight: 76 kDa. But note that when used against the reduced protein identifies bands at 70 Kd, 52 Kd, and a series of further cleaved active forms. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-Calpastatin antibody 图像

    • ICC/IF image of ab28252 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28252, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • All lanes : Anti-Calpastatin antibody (ab28252) at 1/5000 dilution

      Lane 1 : Mouse skeletal tissue muscle lysate
      Lane 2 : Mouse skeletal tissue muscle lysate
      Lane 3 : Mouse skeletal tissue muscle lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      HRP-conjugated Goat IgG to Rabbit
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 76 kDa
      Observed band size : 25,35,74 kDa (why is the actual band size different from the predicted?)

      This image is courtesy of an anonymous Abreview.

      See Abreview

    Anti-Calpastatin antibody (ab28252)参考文献

    This product has been referenced in:
    • Cunha TF  et al. Aerobic exercise training upregulates skeletal muscle calpain and ubiquitin-proteasome systems in healthy mice. J Appl Physiol 112:1839-46 (2012). Read more (PubMed: 22461440) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Sample Mouse Tissue lysate - other (Cytosolic fraction from skeletal muscle)
    Gel Running Conditions Reduced Denaturing (10%)
    Loading amount 20 µg
    Specification Cytosolic fraction from skeletal muscle
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 24 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Tissue lysate - other (Cytosolic Fractions from Skeletal Muscle)
    Gel Running Conditions Reduced Denaturing (10%)
    Loading amount 20 µg
    Specification Cytosolic Fractions from Skeletal Muscle
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 04 2016

    Application Western blot
    Sample Mouse Tissue lysate - whole (Skeletal muscle (AT))
    Loading amount 50 µg
    Specification Skeletal muscle (AT)
    Gel Running Conditions Reduced Denaturing (8% Gel)
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Dr. G.K Sakellariou

    Verified customer

    提交于 Sep 29 2012

    Thank you for submitting an Abreview for AB28252 in mouse.
    I am sorry to hear that this product to did perform to expectations when used in an application and species which we have listed on our datasheet. In reviewing the details of your review I...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (skeletal muscle)
    Loading amount 30 µg
    Specification skeletal muscle
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    提交于 Jan 07 2011

    Thank you for your enquiry. Yes, the antibodies can be used in IHC-P and I have updated the datasheet accordingly. Please use them at an assay dependent dilution using heat-mediated antigen retrieval in citrate buffer pH 6.0. I would recommend a s...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"